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PXD021622

PXD021622 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMapping glycan-mediated galectin-3 interactions by live cell proximity labeling
DescriptionGalectin-3 is a glycan-binding protein (GBP) that binds β -galactoside glycan structures to orchestrate a variety of important biological events, including the activation of hepatic stellate cells and regulating immunological responses. While the requisite glycan epitopes needed to bind galectin-3 have long been elucidated, the cellular glycoproteins that bear these glycan signatures remain unknown. Given the importance of the three-dimensional arrangement of glycans in dictating GBP interactions, strategies that allow the identification of GBP receptors in live cells, where the native glycan presentation and glycoprotein expression are preserved, possess significant advantages over static and artificial systems. Here, we describe the integration of a proximity labeling method and quantitative mass spectrometry to map the glycan and glycoprotein interactors for galectin-3 in live human hepatic stellate cells and peripheral blood mononuclear cells. Understanding the identity of the glycoproteins and defining the structures of the glycans will empower efforts to design and develop selective therapeutics to mitigate galectin-3-mediated biological events.
HostingRepositoryPRIDE
AnnounceDate2021-06-16
AnnouncementXMLSubmission_2021-06-15_22:33:01.865.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEugene Joeh
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-22 06:47:24ID requested
12021-06-15 22:33:02announced
Publication List
Joeh E, O'Leary T, Li W, Hawkins R, Hung JR, Parker CG, Huang ML, Mapping glycan-mediated galectin-3 interactions by live cell proximity labeling. Proc Natl Acad Sci U S A, 117(44):27329-27338(2020) [pubmed]
Keyword List
submitter keyword: galectins, proteomics, proximity labeling
Contact List
Mia L. Huang
contact affiliationDepartment of Molecular Medicine Department of Chemistry The Scripps Research Institute
contact emailmiahuang@scripps.edu
lab head
Eugene Joeh
contact affiliationThe Scripps Research Institute
contact emailejoeh@scripps.edu
dataset submitter
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