PXD021612 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A suite of tools for the study of tryptophan C-mannosylation - additional point mutants |
Description | Tryptophan C-mannosylation is an unusual co-translational protein modification performed by metazoans and apicomplexan parasites. The prevalence of this modification and its biological function is poorly understood, with progress being hampered by a dearth of tools for its study. Here, we engineer of a simple yeast system to enable the production of a diverse array of proteins with and without the modification to interrogate its effect on protein stability and function. This system also facilitated mutagenesis studies, to elucidate what features of the glycosyltransferase and its substrates are crucial for catalysis, and expedited the generation and thorough characterisation of monoclonal antibodies against the protein modification. These antibodies empowered proteomic analyses of the brain C-glycome by enriching for peptides possessing tryptophan C-mannosylation, which revealed new modification sites on difficult-to-study proteins throughout the secretory pathway on both conventional and non-canonical consensus sequences. |
HostingRepository | PRIDE |
AnnounceDate | 2020-11-16 |
AnnouncementXML | Submission_2020-11-16_14:03:43.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Nichollas Scott |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | complex glycosylation |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-22 02:00:33 | ID requested | |
⏵ 1 | 2020-11-16 14:03:43 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: C-mannosylation, glycosylation |
Contact List
Ethan Goddard-Borger |
contact affiliation | Associate Professor Laboratory Head The Walter and Eliza Hall Institute 1G Royal Parade Parkville, VIC, Australia 3052 |
contact email | goddard-borger.e@wehi.edu.au |
lab head | |
Nichollas Scott |
contact affiliation | University of Melbourne |
contact email | nichollas.scott@unimelb.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021612
- Label: PRIDE project
- Name: A suite of tools for the study of tryptophan C-mannosylation - additional point mutants