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PXD021612 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA suite of tools for the study of tryptophan C-mannosylation - additional point mutants
DescriptionTryptophan C-mannosylation is an unusual co-translational protein modification performed by metazoans and apicomplexan parasites. The prevalence of this modification and its biological function is poorly understood, with progress being hampered by a dearth of tools for its study. Here, we engineer of a simple yeast system to enable the production of a diverse array of proteins with and without the modification to interrogate its effect on protein stability and function. This system also facilitated mutagenesis studies, to elucidate what features of the glycosyltransferase and its substrates are crucial for catalysis, and expedited the generation and thorough characterisation of monoclonal antibodies against the protein modification. These antibodies empowered proteomic analyses of the brain C-glycome by enriching for peptides possessing tryptophan C-mannosylation, which revealed new modification sites on difficult-to-study proteins throughout the secretory pathway on both conventional and non-canonical consensus sequences.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNichollas Scott
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListcomplex glycosylation
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-22 02:00:33ID requested
12020-11-16 14:03:43announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: C-mannosylation, glycosylation
Contact List
Ethan Goddard-Borger
contact affiliationAssociate Professor Laboratory Head The Walter and Eliza Hall Institute 1G Royal Parade Parkville, VIC, Australia 3052
contact emailgoddard-borger.e@wehi.edu.au
lab head
Nichollas Scott
contact affiliationUniversity of Melbourne
contact emailnichollas.scott@unimelb.edu.au
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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