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PXD021602-1

PXD021602 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCell type-selective secretome profiling in vivo
DescriptionSecreted polypeptides are a fundamental biochemical axis of intercellular and endocrine communication. However, a global understanding of composition and dynamics of cellular secretomes in intact mammalian organisms has been lacking. Here, we introduce a proximity biotinylation strategy that enables labeling, detection, and enrichment of secreted polypeptides in a cell type-selective manner in mice. We generate a proteomic atlas of hepatocyte, myocyte, pericyte, and myeloid cell secretomes by direct purification of biotinylated secreted polypeptides from blood. Our secretome dataset validates known cell type-protein pairs, reveals secreted polypeptides that distinguish between cell types, and identifies new cellular sources for classical plasma proteins. Lastly, we uncover a dynamic and previously undescribed nutrient-dependent reprogramming of the hepatocyte secretome characterized by increased unconventional secretion of the cytosolic enzyme BHMT. This secretome profiling strategy enables dynamic and cell-type dissection of the plasma proteome and the secreted polypeptides that mediate intercellular signaling.
HostingRepositoryPRIDE
AnnounceDate2020-12-07
AnnouncementXMLSubmission_2020-12-06_23:22:44.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNicholas Riley
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListbiotinylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-21 23:14:32ID requested
12020-12-06 23:22:45announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Plasma, mice, LC-MS/MS, TurboID
Contact List
Jonathan Z. Long
contact affiliationAssistant Professor of Pathology Stanford ChEM-H Institute Scholar Stanford University
contact emailjzlong@stanford.edu
lab head
Nicholas Riley
contact affiliationStanford University
contact emailriley.nicholasm@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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