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PXD021548-1

PXD021548 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProtein turnover rates within mitochondrial complexes in mitochondrial disease patients and in a DNAJC30 knock-out cell line
DescriptionWe identified DNAJC30 as a key protein in the maintenance of functional NADH:ubiquinone oxidoreductase (complex I). To determine the turnover rates of modules in complex I and other mitochondrial complexes, we pulsed both patient and control fibroblast cell lines and a DNAJC30 knock-out HEK and control HEK cell line over 12 hours with heavy amino acids (PulseSILAC) followed by mass spectrometry.
HostingRepositoryPRIDE
AnnounceDate2021-09-09
AnnouncementXMLSubmission_2021-09-09_07:50:55.210.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIlka Wittig
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-17 16:01:07ID requested
12021-09-09 07:50:55announced
Publication List
Stenton SL, Sheremet NL, Catarino CB, Andreeva NA, Assouline Z, Barboni P, Barel O, Berutti R, Bychkov I, Caporali L, Capristo M, Carbonelli M, Cascavilla ML, Charbel Issa P, Freisinger P, Gerber S, Ghezzi D, Graf E, Heidler J, Hempel M, Heon E, Itkis YS, Javasky E, Kaplan J, Kopajtich R, Kornblum C, Kovacs-Nagy R, Krylova TD, Kunz WS, La Morgia C, Lamperti C, Ludwig C, Malacarne PF, Maresca A, Mayr JA, Meisterknecht J, Nevinitsyna TA, Palombo F, Pode-Shakked B, Shmelkova MS, Strom TM, Tagliavini F, Tzadok M, van der Ven AT, Vignal-Clermont C, Wagner M, Zakharova EY, Zhorzholadze NV, Rozet JM, Carelli V, Tsygankova PG, Klopstock T, Wittig I, Prokisch H, Impaired complex I repair causes recessive Leber's hereditary optic neuropathy. J Clin Invest, 131(6):(2021) [pubmed]
Keyword List
submitter keyword: LC-MSMS, complexome profiling, oxidative phosphorylation system, complex I maintenance, complex I assembly, protein turnover
Contact List
Holger Prokisch
contact affiliationResearch Group Mitochondrial Disorders, Institute of Human Genetics, Technische Universität München, München, Germany
contact emailprokisch@helmholtz-muenchen.de
lab head
Ilka Wittig
contact affiliationFunctional Proteomics, Goethe University, Frankfurt am Main , Germany
contact emailwittig@med.uni-frankfurt.de
dataset submitter
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