PXD021533 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | tRNA 3' shortening by LCCR4 as a response to stress in Trypanosoma brucei |
Description | Sensing of environmental cues is crucial for cell survival. To adapt to changes in their surroundings cells need to tightly control the repertoire of genes expressed at any time. Regulation of translation is key, especially in organisms in which transcription is hardly controlled, like Trypanosoma brucei. In this study we describe the shortening of the bulk of the cellular tRNAs during stress at the expense of the conserved 3’ CCA-tail. This tRNA shortening is specific for nutritional stress and renders tRNAs unsuitable substrates for translation. We uncovered the nuclease LCCR4 (Tb927.4.2430), a homologue of the conserved deadenylase Ccr4, as being responsible for tRNA trimming. Once optimal growth conditions are restored tRNAs are rapidly repaired by the trypanosome tRNA nucleotidyltransferase thus rendering the recycled tRNAs amenable for translation. This mechanism represents a fast and efficient way to repress translation during stress, allowing quick reactivation with a low energy input. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-04 |
AnnouncementXML | Submission_2021-03-04_12:54:00.097.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Marina Cristodero |
SpeciesList | scientific name: Trypanosoma brucei; NCBI TaxID: 5691; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-17 03:51:59 | ID requested | |
⏵ 1 | 2021-03-04 12:54:00 | announced | |
Publication List
Cristodero M, Brogli R, Joss O, Schimanski B, Schneider A, Polacek N, tRNA 3' shortening by LCCR4 as a response to stress in Trypanosoma brucei. Nucleic Acids Res, 49(3):1647-1661(2021) [pubmed] |
Keyword List
submitter keyword: Ccr4, tRNA, stress, brucei, translation |
Contact List
Norbert Polacek |
contact affiliation | Department of Chemistry and Biochemistry University of Bern Switzerland |
contact email | norbert.polacek@dcb.unibe.ch |
lab head | |
Marina Cristodero |
contact affiliation | Department of Chemistry and Biochemistry University of Bern |
contact email | marina.cristodero@dcb.unibe.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD021533
- Label: PRIDE project
- Name: tRNA 3' shortening by LCCR4 as a response to stress in Trypanosoma brucei