PXD021533

PXD021533 is an original dataset announced via ProteomeXchange.

Title	tRNA 3' shortening by LCCR4 as a response to stress in Trypanosoma brucei
Description	Sensing of environmental cues is crucial for cell survival. To adapt to changes in their surroundings cells need to tightly control the repertoire of genes expressed at any time. Regulation of translation is key, especially in organisms in which transcription is hardly controlled, like Trypanosoma brucei. In this study we describe the shortening of the bulk of the cellular tRNAs during stress at the expense of the conserved 3’ CCA-tail. This tRNA shortening is specific for nutritional stress and renders tRNAs unsuitable substrates for translation. We uncovered the nuclease LCCR4 (Tb927.4.2430), a homologue of the conserved deadenylase Ccr4, as being responsible for tRNA trimming. Once optimal growth conditions are restored tRNAs are rapidly repaired by the trypanosome tRNA nucleotidyltransferase thus rendering the recycled tRNAs amenable for translation. This mechanism represents a fast and efficient way to repress translation during stress, allowing quick reactivation with a low energy input.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_12:54:00.097.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marina Cristodero
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-17 03:51:59	ID requested
⏵ 1	2021-03-04 12:54:00	announced

Cristodero M, Brogli R, Joss O, Schimanski B, Schneider A, Polacek N, tRNA 3' shortening by LCCR4 as a response to stress in Trypanosoma brucei. Nucleic Acids Res, 49(3):1647-1661(2021) [pubmed]

submitter keyword: Ccr4, tRNA, stress, brucei, translation

Norbert Polacek
contact affiliation	Department of Chemistry and Biochemistry University of Bern Switzerland
contact email	norbert.polacek@dcb.unibe.ch
lab head
Marina Cristodero
contact affiliation	Department of Chemistry and Biochemistry University of Bern
contact email	marina.cristodero@dcb.unibe.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD021533
     1. Label: PRIDE project
     2. Name: tRNA 3' shortening by LCCR4 as a response to stress in Trypanosoma brucei