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PXD021521-1

PXD021521 is an original dataset announced via ProteomeXchange.

Dataset Summary
Title In-depth analysis of proteomic and genomic fluctuations during the time course of human embryonic stem cells directed differentiation into beta cells
DescriptionPluripotent stem cells (PSC) endocrine differentiation at a large scale allows sampling of transcriptome and proteome with phosphoproteome (proteoform) at specific time points. We describe the dynamic time course of changes in cells undergoing directed beta cell differentiation and show target proteins or previously unknown phosphorylation of critical proteins in pancreas development, NKX6.1, and Chromogranin A (CHGA). We describe fluctuations in the correlation between gene expression, its protein abundance, and phosphorylation, which follow differentiation protocol perturbations of cell fates at all stages to id entify proteoform profiles. Our computational modeling recognizes outliers on a phenomic landscape of endocrine differentiation, and we outline several new biological pathways involved. We also suggest that non correlating proteins abundances or new phosphorylation motifs of NKX6.1 and CHGA point to new signaling pathways that may play an essential role in beta cell development.
HostingRepositoryMassIVE
AnnounceDate2021-10-18
AnnouncementXMLSubmission_2021-10-18_09:17:33.970.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBogdan Budnik
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListOxidation; Deamidated; TMT6plex; Carbamidomethyl
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-16 11:43:01ID requested
12021-10-18 09:17:34announced
Publication List
no publication
Keyword List
submitter keyword: Pluripotent stem cells, beta cell differentiation, proteogenomics, phosphoproteomics
Contact List
Dmitry Shvartsman
contact affiliationHarvard University
contact emaildshvartsman@cellariabio.com
lab head
Bogdan Budnik
contact affiliationHarvard University
contact emailbbudnik@mcb.harvard.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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