PXD021494-1

PXD021494 is an original dataset announced via ProteomeXchange.

Title	New antiviral small molecule inhibitors disturb interactions between cellular HSP70 and virus proteins
Description	RNA virus outbreaks such as severe acute respiratory syndrome coronavirus 2 (SARS-CoV2), Ebola virus (EBOV) and Zika virus (ZIKV) causing worldwide health emergencies have highlighted the urgent need of new antiviral strategies. Upon outbreaks with new, unmet viruses where knowledge of virus biology is limited, targeting host cell pathways supporting viral replication is an attractive approach for development of antiviral compounds. Here, we present a strategy to identify novel host-targeted small molecule inhibitors using image-based phenotypic antiviral assay followed by characterization of structure-activity-relationship, mechanism-of-action and molecular targets of the novel antiviral compound using thermal proteome profiling.
HostingRepository	PRIDE
AnnounceDate	2021-01-04
AnnouncementXML	Submission_2021-01-04_07:31:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rozbeh Jafari
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-15 09:31:33	ID requested
⏵ 1	2021-01-04 07:31:27	announced

Tampere M, Pettke A, Salata C, Wallner O, Koolmeister T, Cazares-K, ö, rner A, Visnes T, Hesselman MC, Kunold E, Wiita E, Kalder, é, n C, Lightowler M, Jemth AS, Lehti, ö J, Rosenquist Å, Warpman-Berglund U, Helleday T, Mirazimi A, Jafari R, Puumalainen MR, Novel Broad-Spectrum Antiviral Inhibitors Targeting Host Factors Essential for Replication of Pathogenic RNA Viruses. Viruses, 12(12):(2020) [pubmed]

submitter keyword: LC-MSMS, TPP, 2D-TPP, antiviral compound, virus proteins

Rozbeh Jafari
contact affiliation	Department of Oncology-Pathology (OnkPat) (PI)
contact email	rozbeh.jafari@ki.se
lab head
Rozbeh Jafari
contact affiliation	Karolinska Institutet
contact email	rozbeh.jafari@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD021494

PRIDE project URI

• PRIDE
  1. PXD021494
     1. Label: PRIDE project
     2. Name: New antiviral small molecule inhibitors disturb interactions between cellular HSP70 and virus proteins