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PXD021372-2

PXD021372 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative phosphoproteomic analysis of raf22raf36 in Arabidopsis
DescriptionA phytohormone abscisic acid (ABA) has a major role in abiotic stress responses in plants, and subclass III SNF1-related protein kinase 2 (SnRK2) mediates ABA signaling. In this study, we identified Raf36, a group C Raf-like protein kinase in Arabidopsis, as an interacting protein with SnRK2. A series of reverse genetic and biochemical analyses revealed that Raf36 negatively regulates ABA responses and is directly phosphorylated by SnRK2s. In addition, we found that Raf22, another C-type Raf-like kinase, functions partially redundantly with Raf36 to regulate ABA responses. Comparative phosphoproteomic analysis using Arabidopsis wild-type and raf22raf36-1 plants indicate proteins that are phosphorylated downstream of Raf36 and Raf22 in planta. Together, these results reveal a novel subsection of ABA-responsive phosphosignaling pathways branching from SnRK2.
HostingRepositoryjPOST
AnnounceDate2021-05-15
AnnouncementXMLSubmission_2022-09-18_02:28:10.667.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTaishi Umezawa
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListS-carboxamidomethyl-L-cysteine; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine; L-methionine sulfoxide
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-09 01:34:30ID requested
12021-05-14 23:34:38announced
22022-09-18 02:28:11announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: phosphoproteome, Raf, Arabidopsis, abscisic acid
Contact List
Taishi Umezawa
lab head
Taishi Umezawa
contact affiliationTokyo University of Agriculture and Technology
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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