PXD021370 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Tick (Ixodes ricinus) salivary antigens by LC-MS/MS |
Description | Here, we challenged rabbits with repeated feeding of Ixodes ricinus adults and observed the formation of specific antibodies against several tick salivary proteins. To identify the salivary antigens, isolated immunoglobulins from repeatedly infested rabbits were utilised for a pull-down from the saliva of pilocarpine-treated ticks. Eluted antigens were separated on 1D SDS-PAGE and analysed by peptide mass fingerprinting. To increase the authenticity of immunogens identified, we also performed, for the first time, de novo assembly of the sialome from I. ricinus females fed for six days, a timepoint used for pilocarpine-salivation. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-09_08:29:19.110.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Per Haberkant |
SpeciesList | scientific name: Ixodes ricinus; NCBI TaxID: 34613; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-09-09 01:04:49 | ID requested | |
⏵ 1 | 2021-09-09 08:29:19 | announced | |
Publication List
Perner J, Helm D, Haberkant P, Hatalova T, Kropackova S, Ribeiro JM, Kopacek P, The Central Role of Salivary Metalloproteases in Host Acquired Resistance to Tick Feeding. Front Cell Infect Microbiol, 10():563349(2020) [pubmed] |
Keyword List
submitter keyword: ticks, saliva, antigen, immunoprecipitation |
Contact List
Jan Perner |
contact affiliation | Institute of Parasitology, Biology Centre, Ceske Budejovice |
contact email | perner@paru.cas.cz |
lab head | |
Per Haberkant |
contact affiliation | European Molecular Biology Laboratory (EMBL) |
contact email | per.haberkant@embl.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021370
- Label: PRIDE project
- Name: Tick (Ixodes ricinus) salivary antigens by LC-MS/MS