PXD021370

PXD021370 is an original dataset announced via ProteomeXchange.

Title	Tick (Ixodes ricinus) salivary antigens by LC-MS/MS
Description	Here, we challenged rabbits with repeated feeding of Ixodes ricinus adults and observed the formation of specific antibodies against several tick salivary proteins. To identify the salivary antigens, isolated immunoglobulins from repeatedly infested rabbits were utilised for a pull-down from the saliva of pilocarpine-treated ticks. Eluted antigens were separated on 1D SDS-PAGE and analysed by peptide mass fingerprinting. To increase the authenticity of immunogens identified, we also performed, for the first time, de novo assembly of the sialome from I. ricinus females fed for six days, a timepoint used for pilocarpine-salivation.
HostingRepository	PRIDE
AnnounceDate	2021-09-09
AnnouncementXML	Submission_2021-09-09_08:29:19.110.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Per Haberkant
SpeciesList	scientific name: Ixodes ricinus; NCBI TaxID: 34613; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-09 01:04:49	ID requested
⏵ 1	2021-09-09 08:29:19	announced

Perner J, Helm D, Haberkant P, Hatalova T, Kropackova S, Ribeiro JM, Kopacek P, The Central Role of Salivary Metalloproteases in Host Acquired Resistance to Tick Feeding. Front Cell Infect Microbiol, 10():563349(2020) [pubmed]

submitter keyword: ticks, saliva, antigen, immunoprecipitation

Jan Perner
contact affiliation	Institute of Parasitology, Biology Centre, Ceske Budejovice
contact email	perner@paru.cas.cz
lab head
Per Haberkant
contact affiliation	European Molecular Biology Laboratory (EMBL)
contact email	per.haberkant@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD021370

PRIDE project URI

• PRIDE
  1. PXD021370
     1. Label: PRIDE project
     2. Name: Tick (Ixodes ricinus) salivary antigens by LC-MS/MS