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PXD021276-1

PXD021276 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleeEF2K inhibition synergizes with Glutaminase and eIF4EBP1 inhibitors to suppress triple-negative breast cancer cell growth; impact on the proteome
DescriptionThe eukaryotic elongation factor-2 kinase, eEF2K, restricts protein translation elongation, and was identified as a potential therapeutic target for diverse types of cancers including triple negative breast cancer (TNBC; PMID: 25330770). We have recently found that inhibition of eEF2K synergizes with depletion of eukaryotic translation initiation factor 4E-binding protein 1 (eIF4EBP1; 4E-BP1), a suppressor of eukaryotic protein translation initiation factor 4E (eIF4E), leading to effective growth suppression of TNBC cells in culture. We performed LC-MS/MS analysis following depletion of eEF2K and/or 4EBP in a TNBC cell line, Hs578t (YoungJun Ju & Eldad Zacksenhaus, manuscript in revisions). Depletion of these factors had overlapping effects on the proteome with the highest impact on Collagen containing extracellular matrix (e.g. COL1A1). The complete LC-MS/MS data sets (control vehicle; eEF2K-depletion, 4EBP1-depletion; combined eEF2k plus 4EBP1-depletion) are provided herein.
HostingRepositoryMassIVE
AnnounceDate2020-11-16
AnnouncementXMLSubmission_2020-11-16_06:52:26.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterYoungJun Ju
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListOxidation; Carbamidomethyl; Acetyl; TMT6plex
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-09-02 10:25:49ID requested
12020-11-16 06:52:26announced
Publication List
no publication
Keyword List
submitter keyword: eEF2K, 4EBP1, translation initiation, translation elongation, cancer therapy, triple negative breast cancer
Contact List
YoungJun Ju
contact affiliationUHN
contact emailyju@lakeheadu.ca
lab head
Eldad Zacksenhaus
contact affiliationUHN
contact emaileldad.zacksenhaus@utoronto.ca
lab head
YoungJun Ju
contact affiliationUHN
contact emailyju@lakeheadu.ca
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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