PXD021276

PXD021276 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	eEF2K inhibition synergizes with Glutaminase and eIF4EBP1 inhibitors to suppress triple-negative breast cancer cell growth; impact on the proteome
Description	The eukaryotic elongation factor-2 kinase, eEF2K, restricts protein translation elongation, and was identified as a potential therapeutic target for diverse types of cancers including triple negative breast cancer (TNBC; PMID: 25330770). We have recently found that inhibition of eEF2K synergizes with depletion of eukaryotic translation initiation factor 4E-binding protein 1 (eIF4EBP1; 4E-BP1), a suppressor of eukaryotic protein translation initiation factor 4E (eIF4E), leading to effective growth suppression of TNBC cells in culture. We performed LC-MS/MS analysis following depletion of eEF2K and/or 4EBP in a TNBC cell line, Hs578t (YoungJun Ju & Eldad Zacksenhaus, manuscript in revisions). Depletion of these factors had overlapping effects on the proteome with the highest impact on Collagen containing extracellular matrix (e.g. COL1A1). The complete LC-MS/MS data sets (control vehicle; eEF2K-depletion, 4EBP1-depletion; combined eEF2k plus 4EBP1-depletion) are provided herein.
HostingRepository	MassIVE
AnnounceDate	2020-11-16
AnnouncementXML	Submission_2020-11-16_06:52:26.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	YoungJun Ju
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl; Acetyl; TMT6plex
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-09-02 10:25:49	ID requested
⏵ 1	2020-11-16 06:52:26	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: eEF2K, 4EBP1, translation initiation, translation elongation, cancer therapy, triple negative breast cancer

submitter keyword: eEF2K, 4EBP1, translation initiation, translation elongation, cancer therapy, triple negative breast cancer

Contact List

YoungJun Ju
contact affiliation	UHN
contact email	yju@lakeheadu.ca
lab head
Eldad Zacksenhaus
contact affiliation	UHN
contact email	eldad.zacksenhaus@utoronto.ca
lab head
YoungJun Ju
contact affiliation	UHN
contact email	yju@lakeheadu.ca
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086059/