PXD021204 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of LARP6 depletion impact on the proteome of MDA-MB231 breast cancer cells |
Description | We analysed the impact of LARP6 depletion on the proteome of actively growing MDA-MB231 breast cancer cells by SILAC. For this purpose, Light (L) SILAC-labelled MDA-MB231 cells were treated with non-targeting control (NT) or two independent LARP6 siRNA (18i & 97i) for 72 hrs, before lysis in 4% SDS, 100mM Tris/HCl pH 7.5. In parallel, Heavy (H)SILAC labelled non-transfected MDA-MB231 cells were grown and lysed similarly. Each L labeled lysate was then mixed with an equal amount of H labelled lysate. Mixing of samples to the same H standard therefore allowed cross-comparison of different siRNA treatments from separate runs. |
HostingRepository | PRIDE |
AnnounceDate | 2020-10-20 |
AnnouncementXML | Submission_2020-10-19_22:28:59.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Maria Dermit Salazar |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-31 01:07:58 | ID requested | |
⏵ 1 | 2020-10-19 22:29:00 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: LARP6 |
SILAC |
MDA-MB231 |
Breast cancer |
Orbitrap |
Contact List
Faraz Mardakheh |
contact affiliation | Centre for Cancer Cell & Molecular Biology Barts Cancer Institute Queen Mary University of London Charterhouse Square London EC1M 6BQ The United Kingdom. |
contact email | f.mardakheh@qmul.ac.uk |
lab head | |
Maria Dermit Salazar |
contact affiliation | qmul |
contact email | mariaprideproteomics@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/10/PXD021204 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD021204
- Label: PRIDE project
- Name: Analysis of LARP6 depletion impact on the proteome of MDA-MB231 breast cancer cells