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PXD021086-1

PXD021086 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteo-metabolomic study of olive (cv. ‘Chondrolia Chalkidikis’) drupe development and maturation
DescriptionOlive (Olea europaea L.) is one of the most economically relevant tree crops in the Mediterranean basin. In this study, a comparative proteomic along with metabolomic-wide investigation was carried out on drupes of Greek olive cultivar 'Chondrolia Chalkidikis', collected across six developmental stages (S), namely seed development (S1, S2), mesocarp development (S3, S4, S5) and full maturation (S6). These stages were first characterized through the dynamics of fruit weight, dimensions and color parameters such as lightness, redness and yellowness. Combined gas chromatography–mass spectrometry and reversed–phase liquid chromatography quadrupole–time–of–flight mass spectrometry (RPLC–QToF–MS) procedures quantified 47 primary (e.g. allose, galactose, quinic acid, sorbitol, stearic acid) and 21 secondary (e.g. elenolic acid, oleacin, rutin, luteolin, hydroxytyrosol) metabolites in mesocarp samples during development. Protein analysis via nano–LC coupled to HDAM Orbitrap mass spectrometer, identified 3258 proteins from which the 350 were differentially accumulated between the final maturation stages (S5 and S6). Olive genome-based functional annotation showed that the largest proportion of identified proteins were involved in primary metabolism [i.e. lipoxygenases (LOX1/5)], energy [i.e. ferredoxin NADP+ reductase (FNR)], signal transduction [i.e. serine/threonine kinases (SAPK2, SRK2A, STK), transcription [i.e. elongation factor 2 (EEF2)] and protein destination [i.e. serine carboxypeptidase (SCPL)]. This investigation provides a reference framework for further nutritional and breeding studies, also allowing cross comparison among other olive cultivars.
HostingRepositoryPRIDE
AnnounceDate2021-11-25
AnnouncementXMLSubmission_2021-11-25_07:42:58.267.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMartina Samiotaki
SpeciesList scientific name: Olea europaea (Common olive); NCBI TaxID: 4146;
ModificationListmonohydroxylated residue; acetylated residue; dehydromethionine; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-24 07:11:12ID requested
12021-11-25 07:42:58announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: primary metabolism, secondary metabolism, olive drupe development, maturity, proteomics.
Contact List
Athanassios Molassiotis
contact affiliationSchool of Agriculture Aristotle University of Thessaloniki541 24 Thessaloniki, Greece
contact emailamolasio@agro.auth.gr
lab head
Martina Samiotaki
contact affiliationProtein Analysis Laboratory B.S.R.C. "Alexander Fleming", Alexander Fleming Street 34 16672, Vari, Greece
contact emailsamiotaki@fleming.gr
dataset submitter
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