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PXD021029-1

PXD021029 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDissection of anti-tumor activity of histone deacetylase inhibitor SAHA in NPC cells via quantitative phosphoproteomics
DescriptionSuberoylanilide hydroxamic acid (SAHA), as a pan HDAC inhibitor, has been approved for clinical use and it has been demonstrated to treat solid tumor. Nevertheless, the mechanism underlying the therapeutic effects of SAHA on tumors are still not fully understood. Protein phosphorylation is one of the most important regulatory events in cells, regulating primary biological processes, such as cell division, growth, migration, differentiation, and intercellular communication. The impact of SAHA treatment on cellular phosphorylation and signaling pathways might contribute to our understanding on its therapeutic mechanism. For this purpose, by combining tandem mass tags(TMT)-based quantitative proteomics and titanium dioxide-based phosphopeptide enrichment, we for the first time comprehensively identified and quantified protein phosphorylation in NPC cells toward SAHA treatment. we identified 7430 phosphorylation sites on 2456 phosphoproteins in the NPC cell line 5-8F, of which 1176 phosphorylation sites on 528 phosphoproteins were significantly expressed upon SAHA treatment. Biological analysis show that SAHA can influence several biological processes including mRNA/DNA processing, cell cycle. Furthermore, signaling pathway analysis demonstrate that SAHA can activate cell cycle regulation related pathway such as p53 signalling pathway, and inactivate energetic pathway such as AMPK pathway etc. Overall, our study presents evidence at a phosphoproteomic level that SAHA can inhibit NPC cell growth by regulating the cell cycle and cell energy metabolism. Our findings will contribute to elucidation of the mechanisms through which SAHA exerts its anticancer effects.
HostingRepositoryiProX
AnnounceDate2020-08-20
AnnouncementXMLSubmission_2020-08-20_19:31:19.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaoyu Li
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-20 19:29:38ID requested
12020-08-20 19:31:20announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: HDAC inhibitor, SAHA, quantitative phosphoproteomics
Contact List
Yongheng Chen
contact affiliationNHC Key Lab of Cancer Proteomics, Xiangya Hospital, Central South University
contact emailyonghenc@163.com
lab head
Maoyu Li
contact affiliationCentral South University
contact emailmaoyuli@126.com
dataset submitter
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iProX dataset URI