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PXD021026-1

PXD021026 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics characterisation of the L929 cell supernatant and its role in BMDM differentiation
DescriptionBone marrow-derived macrophages (BMDMs) are a key model system for studying macrophage biology in vitro. Commonly used methods to differentiate macrophages from bone marrow are treatment with either recombinant M-CSF or the supernatant of L929 cells, which secrete M-CSF. However, little is known about the composition of L929 conditioned media (CM) and how it affects BMDM phenotype. Here, we used quantitative mass spectrometry to characterise the kinetics of protein secretion from L929 cells over a two-week period. While M-CSF is very abundant in L929 CM, we identified several other immune-regulatory proteins at surprisingly high abundance. L929 CM induced a slightly pre-activated phenotype and the expression of a number of known innate immune proteins in macrophages. This resource will be valuable to all researchers using L929 CM for the differentiation of BMDMs.
HostingRepositoryPRIDE
AnnounceDate2021-04-13
AnnouncementXMLSubmission_2021-04-13_06:53:49.113.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMatthias Trost
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion Lumos; Q Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-20 08:42:08ID requested
12021-04-13 06:53:49announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Bone marrow-derived macrophages, L929, secretome, proteomics, differentiation, M-CSF
Contact List
Matthias Trost
contact affiliationNewcastle University Biosciences Institute, Newcastle University, Newcastle upon Tyne, NE24HH, United Kingdom
contact emailmatthias.trost@ncl.ac.uk
lab head
Matthias Trost
contact affiliationNewcastle University
contact emailmatthias.trost@ncl.ac.uk
dataset submitter
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Dataset FTP location
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