<<< Full experiment listing

PXD020856-1

PXD020856 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHarnessing Ionic Selectivity In Acetyltransferase Chemoproteomic Probes
DescriptionChemical proteomics (chemoproteomics) has emerged as a powerful strategy for the high-throughput annotation of protein function. This approach involves using an active site probe to capture an enzyme class of interest from cells, allowing the biological activity of multiple enzymes within that family to be assessed in parallel. Coenzyme A based chemoproteomic probes may have utility in studying the pharmacological interactions of additional enzyme classes, beyond histone acetyltransferases. However, one challenge in applying this approach is how to differentiate a ‘true hit’ from background. Many histone acetyltransferases utilize an ordered binding mechanism, with acetyl-CoA binding first, which allows their chemoproteomic capture to be competed by pre-incubating lysates with acetyl-CoA. However, for acetyltransferases that don’t exhibit this binding mode, or which bind to a different ligand, acetyl-CoA competition may not be evident, limiting our ability to discern selective chemoproteomic enrichment from noise. In this study, two physiochemically distinct capture probes were used to enrich proteins to distinguish true hits from background and identify new targets selectively enriched by CoA-based probes, thus expanding the scope of protein-ligand interactions open to chemoproteomic interrogation.
HostingRepositoryPRIDE
AnnounceDate2021-08-19
AnnouncementXMLSubmission_2021-08-18_22:46:57.964.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD020856
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMichaella Levy
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-11 17:00:54ID requested
12021-08-18 22:46:58announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Acetyltransferase, Affinity Capture, TMT
Contact List
Laurence Florens
contact affiliationStowers Institute for Medical Research
contact emaillaf@stowers.org
lab head
Michaella Levy
contact affiliationStowers Institute for Medical Research
contact emailmly@stowers.org
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD020856
PRIDE project URI
Repository Record List
[ + ]