PXD020849-1

PXD020849 is an original dataset announced via ProteomeXchange.

Title	Structural inventory of native ribosomal ABCE1-43S pre-initiation complex
Description	In eukaryotic translation, the termination and recycling phases are linked to subsequent initiation by persistence of several factors. These comprise the large eIF3 complex, eIF3j (Hcr1 in yeast) and the ATP-binding cassette protein ABCE1 (Rli1 in yeast). The ATPase is mainly active as a recycling factor, but it can remain bound to the dissociated 40S subunit until formation of 43S pre-initiation complexes. However, its functional role and native architectural context remains largely enigmatic. Here, we present an architectural inventory of native yeast and human ABCE1-containing pre-initiation complexes by cryo-EM. We found that ABCE1 was mostly associated with early 43S but also later 48S phases of initiation. It adopted a novel hybrid conformation of its nucleotide binding sites, which was stabilized by the N-terminus of eIF3j. Further, eIF3j occupied the mRNA entry channel via its ultimate C-terminus explaining its antagonistic role with respect to mRNA binding. Moreover, the native human samples provided a near-complete molecular picture of the architecture and sophisticated interaction network of the 43S-bound eIF3 complex and also the eIF2 ternary complex containing the initiator tRNA.
HostingRepository	PRIDE
AnnounceDate	2020-10-16
AnnouncementXML	Submission_2020-10-16_06:36:22.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mia Potocnjak
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-08-11 07:19:27	ID requested
⏵ 1	2020-10-16 06:36:22	announced

Dataset with its publication pending

submitter keyword: Yeast, Translation initiation, ABCE1, ABC-proteins, Ribosome

Roland Beckmann
contact affiliation	Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, University of Munich, Munich, Germany
contact email	beckmann@genzentrum.lmu.de
lab head
Mia Potocnjak
contact affiliation	Gene Center
contact email	potocnjak@genzentrum.lmu.de
dataset submitter

Dataset FTP location

PRIDE project URI

• PRIDE
  1. PXD020849
     1. Label: PRIDE project
     2. Name: Structural inventory of native ribosomal ABCE1-43S pre-initiation complex