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PXD020849-1

PXD020849 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleStructural inventory of native ribosomal ABCE1-43S pre-initiation complex
DescriptionIn eukaryotic translation, the termination and recycling phases are linked to subsequent initiation by persistence of several factors. These comprise the large eIF3 complex, eIF3j (Hcr1 in yeast) and the ATP-binding cassette protein ABCE1 (Rli1 in yeast). The ATPase is mainly active as a recycling factor, but it can remain bound to the dissociated 40S subunit until formation of 43S pre-initiation complexes. However, its functional role and native architectural context remains largely enigmatic. Here, we present an architectural inventory of native yeast and human ABCE1-containing pre-initiation complexes by cryo-EM. We found that ABCE1 was mostly associated with early 43S but also later 48S phases of initiation. It adopted a novel hybrid conformation of its nucleotide binding sites, which was stabilized by the N-terminus of eIF3j. Further, eIF3j occupied the mRNA entry channel via its ultimate C-terminus explaining its antagonistic role with respect to mRNA binding. Moreover, the native human samples provided a near-complete molecular picture of the architecture and sophisticated interaction network of the 43S-bound eIF3 complex and also the eIF2 ternary complex containing the initiator tRNA.
HostingRepositoryPRIDE
AnnounceDate2020-10-16
AnnouncementXMLSubmission_2020-10-16_06:36:22.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMia Potocnjak
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-11 07:19:27ID requested
12020-10-16 06:36:22announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Yeast, Translation initiation, ABCE1, ABC-proteins, Ribosome
Contact List
Roland Beckmann
contact affiliationGene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, University of Munich, Munich, Germany
contact emailbeckmann@genzentrum.lmu.de
lab head
Mia Potocnjak
contact affiliationGene Center
contact emailpotocnjak@genzentrum.lmu.de
dataset submitter
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Dataset FTP location
PRIDE project URI
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