PXD020849 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Structural inventory of native ribosomal ABCE1-43S pre-initiation complex |
Description | In eukaryotic translation, the termination and recycling phases are linked to subsequent initiation by persistence of several factors. These comprise the large eIF3 complex, eIF3j (Hcr1 in yeast) and the ATP-binding cassette protein ABCE1 (Rli1 in yeast). The ATPase is mainly active as a recycling factor, but it can remain bound to the dissociated 40S subunit until formation of 43S pre-initiation complexes. However, its functional role and native architectural context remains largely enigmatic. Here, we present an architectural inventory of native yeast and human ABCE1-containing pre-initiation complexes by cryo-EM. We found that ABCE1 was mostly associated with early 43S but also later 48S phases of initiation. It adopted a novel hybrid conformation of its nucleotide binding sites, which was stabilized by the N-terminus of eIF3j. Further, eIF3j occupied the mRNA entry channel via its ultimate C-terminus explaining its antagonistic role with respect to mRNA binding. Moreover, the native human samples provided a near-complete molecular picture of the architecture and sophisticated interaction network of the 43S-bound eIF3 complex and also the eIF2 ternary complex containing the initiator tRNA. |
HostingRepository | PRIDE |
AnnounceDate | 2020-10-16 |
AnnouncementXML | Submission_2020-10-16_06:36:22.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mia Potocnjak |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-08-11 07:19:27 | ID requested | |
⏵ 1 | 2020-10-16 06:36:22 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Yeast, Translation initiation, ABCE1, ABC-proteins, Ribosome |
Contact List
Roland Beckmann |
contact affiliation | Gene Center and Center for Integrated Protein Science Munich, Department of Biochemistry, University of Munich, Munich, Germany |
contact email | beckmann@genzentrum.lmu.de |
lab head | |
Mia Potocnjak |
contact affiliation | Gene Center |
contact email | potocnjak@genzentrum.lmu.de |
dataset submitter | |
Full Dataset Link List
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020849
- Label: PRIDE project
- Name: Structural inventory of native ribosomal ABCE1-43S pre-initiation complex