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PXD020820-1

PXD020820 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFull geroprotection from brief rapamycin treatment by persistently increased intestinal autophagy
DescriptionThe licensed drug rapamycin has potential to be repurposed for geroprotection. A key challenge is to avoid the adverse side-effects of clinical dosing regimes. Here we show a profound memory effect of brief rapamycin treatment. Brief, early adult treatment extended lifespan in Drosophila to the same degree as lifelong dosing. Lasting memory of earlier rapamycin treatment was mediated by elevated autophagy in enterocytes of the gut, accompanied by increased intestinal spermidine levels and improved structure and function of the ageing intestine. Brief elevation of autophagy itself induced a long-term increase in autophagy. In mice, short-term, 3-month treatment also induced a full memory effect, with enhanced autophagy in Paneth cells, improved Paneth cell architecture and gut barrier function at levels induced by chronic treatment, even 6 months after rapamycin was withdrawn. Past rapamycin treatment also enhanced the regenerative potential of aged intestine in intestinal organoids. Full geroprotective effects of chronic rapamycin treatment can thus be obtained with a brief pulse of the drug.
HostingRepositoryPRIDE
AnnounceDate2022-07-18
AnnouncementXMLSubmission_2022-07-18_05:35:25.302.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterIlian Atanassov
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; N-acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-10 03:46:34ID requested
12022-07-18 05:35:25announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: autophagy, TMT, rapamycin, lifespan, fly, drosophila
Contact List
Linda Partridge
contact affiliationMax Planck Institute for Biology of Ageing, Cologne, Germany. Institute of Healthy Ageing, and Department of Genetics, Evolution and Environment, UCL, London, UK.
contact emailLinda.Partridge@age.mpg.de
lab head
Ilian Atanassov
contact affiliationMax Planck Institute for Biology of Aging
contact emailIlian.Atanassov@age.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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