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PXD020731-1

PXD020731 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleErbB2 drives Yap activation during cardiac regeneration
DescriptionCardiomyocyte (CM) loss after injury results in adverse remodelling and fibrosis, which inevitably lead to heart failure. Neuregulin-ErbB2 and Hippo-Yap signaling pathways are key mediators of CM proliferation and regeneration although the crosstalk between these pathways is unclear. Here, we demonstrate in mice that temporal over-expression (OE) of activated ErbB2 in CMs promotes cardiac regeneration in a heart failure model. Cellularly, OE CMs present an EMT-like regenerative response involving cytoskeletal reprograming, migration, ECM turnover, and displacement. Molecularly, we identified Yap as a critical mediator of ErbB2 signaling. In OE CMs, Yap interacts with nuclear envelope and cytoskeletal components, reflective of the altered mechanic state elicited by ErbB2. Hippo-independent activating phosphorylation on Yap at S352 and S274 were enriched in OE CMs, peaking during metaphase. Viral overexpression of Yap phospho-mutants dampened the proliferative competence of OE CMs. Taken together, we demonstrate a potent ErbB2-mediated Yap mechanosensory signaling involving EMT-like characteristics, resulting in heart regeneration.
HostingRepositoryPRIDE
AnnounceDate2020-08-21
AnnouncementXMLSubmission_2020-08-21_06:17:39.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlon Savidor
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue; monohydroxylated residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-05 02:56:07ID requested
12020-08-21 06:17:39announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: LFQ, DDA, phosphoproteomics, mouse, orbitrap, IP
Contact List
Eldad Tzahor
contact affiliationDepartment of Molecular Cell Biology Weizmann Institute of Science
contact emaileldad.tzahor@weizmann.ac.il
lab head
Alon Savidor
contact affiliationThe Weizmann Institute of Science
contact emailalon.savidor@weizmann.ac.il
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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