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PXD020682-1

PXD020682 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLocal RNA translation controls cell migration and Rab GTPase function
DescriptionNumerous RNAs exhibit specific distribution patterns in mammalian cells. However, the functional and mechanistic consequences are relatively unknown. We investigate here the functional role of RNA localization at cellular protrusions of mesenchymal migrating cells, using as a model the RAB13 RNA, which encodes a GTPase important for vesicle-mediated membrane trafficking. While RAB13 RNA is enriched at peripheral protrusions, the expressed protein is concentrated perinuclearly. By specifically preventing RAB13 RNA localization, we show that peripheral RAB13 translation is not important for the overall distribution of the RAB13 protein, or its ability to associate with membranes, but is required for full activation of the GTPase and for efficient cell migration. This effect is mediated by a co-translational association of RAB13 with the exchange factor RABIF. Our results indicate that RAB13-RABIF association at the periphery is required for directing RAB13 GTPase activity to promote cell migration. Thus, translation of RAB13 in specific subcellular environments imparts the protein with distinct properties and highlights a means of controlling protein function through local RNA translation.
HostingRepositoryMassIVE
AnnounceDate2020-09-08
AnnouncementXMLSubmission_2020-09-08_05:04:21.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterLisa Jenkins
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Fusion ETD
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-08-01 14:36:06ID requested
12020-09-08 05:04:21announced
Publication List
no publication
Keyword List
submitter keyword: Rab13, Rab interactome
Contact List
Lisa Jenkins
contact affiliationNCI, NIH
contact emailjenkinsl@mail.nih.gov
lab head
Lisa Jenkins
contact affiliationNCI
contact emailjenkinsl@mail.nih.gov
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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