PXD020682
PXD020682 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Local RNA translation controls cell migration and Rab GTPase function |
| Description | Numerous RNAs exhibit specific distribution patterns in mammalian cells. However, the functional and mechanistic consequences are relatively unknown. We investigate here the functional role of RNA localization at cellular protrusions of mesenchymal migrating cells, using as a model the RAB13 RNA, which encodes a GTPase important for vesicle-mediated membrane trafficking. While RAB13 RNA is enriched at peripheral protrusions, the expressed protein is concentrated perinuclearly. By specifically preventing RAB13 RNA localization, we show that peripheral RAB13 translation is not important for the overall distribution of the RAB13 protein, or its ability to associate with membranes, but is required for full activation of the GTPase and for efficient cell migration. This effect is mediated by a co-translational association of RAB13 with the exchange factor RABIF. Our results indicate that RAB13-RABIF association at the periphery is required for directing RAB13 GTPase activity to promote cell migration. Thus, translation of RAB13 in specific subcellular environments imparts the protein with distinct properties and highlights a means of controlling protein function through local RNA translation. |
| HostingRepository | MassIVE |
| AnnounceDate | 2020-09-08 |
| AnnouncementXML | Submission_2020-09-08_05:04:21.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Lisa Jenkins |
| SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion ETD |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-08-01 14:36:06 | ID requested | |
| ⏵ 1 | 2020-09-08 05:04:21 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Rab13, Rab interactome |
Contact List
| Lisa Jenkins | |
|---|---|
| contact affiliation | NCI, NIH |
| contact email | jenkinsl@mail.nih.gov |
| lab head | |
| Lisa Jenkins | |
| contact affiliation | NCI |
| contact email | jenkinsl@mail.nih.gov |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085883/ |
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