PXD020431-1

PXD020431 is an original dataset announced via ProteomeXchange.

Title	Mapping sites of O-fucose modification on mouse NOTCH2
Description	The O-fucose modification sites on mouse NOTCH2 were determined by mass spectral glycoproteomic site mapping. The Extracellular Domain of mouse NOTCH2 was produced in HEK293T cells in the presence or absence of the three Fringe enzymes: Lunatic fringe, Manic fringe, or Radical fringe. The protein was purified from the medium, reduced and alkylated, digested with trypsin, chymotrypsin or V8 protease, and the resulting peptides were analyzed by nano-LC mass spectrometry. The majority of the sites were identified using an Agilent model 6340 Ion Trap mass spectrometer. A few sites were identified using a Q-Exactive Plus mass spectrometer.
HostingRepository	PRIDE
AnnounceDate	2020-09-11
AnnouncementXML	Submission_2020-09-11_05:09:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Haltiwanger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	O-fucosyl-L-serine; O-fucosyl-L-threonine; iodoacetamide derivatized residue
Instrument	6340 Ion Trap LC/MS; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-18 18:28:23	ID requested
⏵ 1	2020-09-11 05:09:08	announced

Kakuda S, LoPilato RK, Ito A, Haltiwanger RS, Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2. J Biol Chem, 295(43):14710-14722(2020) [pubmed]

submitter keyword: NOTCH2, Fringe, O-fucose, EGF repeats

Robert S. Haltiwanger
contact affiliation	Complex Carbohydrate Reserach Center Department of Biochemistry and Molecular Biology University of Georgia Athens, GA 30621 USA
contact email	rhalti@uga.edu
lab head
Robert Haltiwanger
contact affiliation	Complex Carbohydrate Research Center University of Georgia
contact email	rhalti@uga.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD020431
     1. Label: PRIDE project
     2. Name: Mapping sites of O-fucose modification on mouse NOTCH2