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PXD020431 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMapping sites of O-fucose modification on mouse NOTCH2
DescriptionThe O-fucose modification sites on mouse NOTCH2 were determined by mass spectral glycoproteomic site mapping. The Extracellular Domain of mouse NOTCH2 was produced in HEK293T cells in the presence or absence of the three Fringe enzymes: Lunatic fringe, Manic fringe, or Radical fringe. The protein was purified from the medium, reduced and alkylated, digested with trypsin, chymotrypsin or V8 protease, and the resulting peptides were analyzed by nano-LC mass spectrometry. The majority of the sites were identified using an Agilent model 6340 Ion Trap mass spectrometer. A few sites were identified using a Q-Exactive Plus mass spectrometer.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterRobert Haltiwanger
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListO-fucosyl-L-serine; O-fucosyl-L-threonine; iodoacetamide derivatized residue
Instrument6340 Ion Trap LC/MS; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-18 18:28:23ID requested
12020-09-11 05:09:08announced
Publication List
Kakuda S, LoPilato RK, Ito A, Haltiwanger RS, Canonical Notch ligands and Fringes have distinct effects on NOTCH1 and NOTCH2. J Biol Chem, 295(43):14710-14722(2020) [pubmed]
Keyword List
submitter keyword: NOTCH2, Fringe, O-fucose, EGF repeats
Contact List
Robert S. Haltiwanger
contact affiliationComplex Carbohydrate Reserach Center Department of Biochemistry and Molecular Biology University of Georgia Athens, GA 30621 USA
contact emailrhalti@uga.edu
lab head
Robert Haltiwanger
contact affiliationComplex Carbohydrate Research Center University of Georgia
contact emailrhalti@uga.edu
dataset submitter
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Dataset FTP location
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