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PXD020419-1

PXD020419 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMammalian telomeres reforged with non-telomeric sequences
DescriptionTelomere is a highly refined system for maintaining the stability of linear chromosomes. Most telomeres rely on simple repetitive sequences and telomerase enzymes, but in some species or telomerase-defective situations, alternative telomere lengthening (ALT) mechanism is utilized to protect chromosomal ends. Telomere loss can induce telomere recombination by which specific sequences can be recruited into telomeres. However, canonical telomeric repeat-based telomeres have been found in mammals. Here, we show that mammalian telomeres can also be completely reconstituted using a non-telomeric unique sequence. We found that a specific subtelomeric element, named as mouse template for ALT (mTALT), is utilized for repairing telomeric DNA damage and composing new telomeric sequences in mouse embryonic stem cells. We found a high-level of non-coding mTALT transcript despite the heterochromatic nature of mTALT-based telomere. After ALT activation, the increased HMGN1, a non-histone chromosomal protein, contributed to maintaining telomere stability by regulating telomeric transcriptions. Our findings reveal novel molecular features of potential telomeric sequences which can reconstitute telomeres during cancer formation and evolution.
HostingRepositoryMassIVE
AnnounceDate2020-12-25
AnnouncementXMLSubmission_2020-12-25_18:59:45.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSunny
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListTMT6plex
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-17 11:04:14ID requested
12020-12-25 18:59:46announced
Publication List
no publication
Keyword List
submitter keyword: telomere, alternative lengthening of telomeres, copy number variation, telomere damage, HMGN1, telomere repeat-containing RNA
Contact List
Junho Lee
contact affiliationSeoul National University
contact emailelegans@snu.ac.kr
lab head
Sunny
contact affiliationSNU
contact emailsunnyshin54@gmail.com
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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