PXD020419
PXD020419 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mammalian telomeres reforged with non-telomeric sequences |
| Description | Telomere is a highly refined system for maintaining the stability of linear chromosomes. Most telomeres rely on simple repetitive sequences and telomerase enzymes, but in some species or telomerase-defective situations, alternative telomere lengthening (ALT) mechanism is utilized to protect chromosomal ends. Telomere loss can induce telomere recombination by which specific sequences can be recruited into telomeres. However, canonical telomeric repeat-based telomeres have been found in mammals. Here, we show that mammalian telomeres can also be completely reconstituted using a non-telomeric unique sequence. We found that a specific subtelomeric element, named as mouse template for ALT (mTALT), is utilized for repairing telomeric DNA damage and composing new telomeric sequences in mouse embryonic stem cells. We found a high-level of non-coding mTALT transcript despite the heterochromatic nature of mTALT-based telomere. After ALT activation, the increased HMGN1, a non-histone chromosomal protein, contributed to maintaining telomere stability by regulating telomeric transcriptions. Our findings reveal novel molecular features of potential telomeric sequences which can reconstitute telomeres during cancer formation and evolution. |
| HostingRepository | MassIVE |
| AnnounceDate | 2020-12-25 |
| AnnouncementXML | Submission_2020-12-25_18:59:45.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Sunny |
| SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
| ModificationList | TMT6plex |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-07-17 11:04:14 | ID requested | |
| ⏵ 1 | 2020-12-25 18:59:46 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: telomere, alternative lengthening of telomeres, copy number variation, telomere damage, HMGN1, telomere repeat-containing RNA |
Contact List
| Junho Lee | |
|---|---|
| contact affiliation | Seoul National University |
| contact email | elegans@snu.ac.kr |
| lab head | |
| Sunny | |
| contact affiliation | SNU |
| contact email | sunnyshin54@gmail.com |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000085772/ |
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