PXD020399-1

PXD020399 is an original dataset announced via ProteomeXchange.

Title	Targeted proteomics measurements for Genetically controlled membrane synthesis in liposomes
Description	Lipid membranes, nucleic acids, proteins, and metabolism are essential for modern cellular life. Synthetic systems emulating the fundamental properties of living cells must therefore be built upon these functional elements. In this work, phospholipid-producing enzymes encoded in a synthetic minigenome are cell-free expressed within liposome compartments. The de novo synthesized metabolic pathway converts precursors into a variety of lipids, including the constituents of the parental liposome. Balanced production of phosphatidylethanolamine and phosphatidylglycerol is realized, owing to transcriptional regulation of the activity of specific genes combined with a metabolic feedback mechanism. Fluorescence-based methods are developed to image the synthesis and membrane incorporation of phosphatidylserine at the single liposome level. Our results provide experimental evidence for DNA-programmed membrane synthesis in a minimal cell model. Strategies are discussed to alleviate current limitations toward more effective liposome growth and self-reproduction.
HostingRepository	PanoramaPublic
AnnounceDate	2021-03-12
AnnouncementXML	Submission_2021-03-12_19:55:31.144.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Foschepoth
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	6460 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2020-07-16 05:11:44	ID requested
⏵ 1	2021-03-12 19:55:31	announced

Blanken D, Foschepoth D, Serr, ã, o AC, Danelon C, Genetically controlled membrane synthesis in liposomes. Nat Commun, 11(1):4317(2020) [pubmed]

submitter keyword: PURE, cell free, synthetic cell, gene expression, lipid biosynthesis

David Foschepoth
contact affiliation	TU Delft
contact email	d.j.foschepoth@tudelft.nl
lab head
David Foschepoth
contact affiliation	TU Delft
contact email	d.j.foschepoth@tudelft.nl
dataset submitter

Panorama Public dataset URI