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PXD020399

PXD020399 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTargeted proteomics measurements for Genetically controlled membrane synthesis in liposomes
DescriptionLipid membranes, nucleic acids, proteins, and metabolism are essential for modern cellular life. Synthetic systems emulating the fundamental properties of living cells must therefore be built upon these functional elements. In this work, phospholipid-producing enzymes encoded in a synthetic minigenome are cell-free expressed within liposome compartments. The de novo synthesized metabolic pathway converts precursors into a variety of lipids, including the constituents of the parental liposome. Balanced production of phosphatidylethanolamine and phosphatidylglycerol is realized, owing to transcriptional regulation of the activity of specific genes combined with a metabolic feedback mechanism. Fluorescence-based methods are developed to image the synthesis and membrane incorporation of phosphatidylserine at the single liposome level. Our results provide experimental evidence for DNA-programmed membrane synthesis in a minimal cell model. Strategies are discussed to alleviate current limitations toward more effective liposome growth and self-reproduction.
HostingRepositoryPanoramaPublic
AnnounceDate2021-03-12
AnnouncementXMLSubmission_2021-03-12_19:55:31.144.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterDavid Foschepoth
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListLabel:13C(6)15N(2); Label:13C(6)15N(4)
Instrument6460 Triple Quadrupole LC/MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-07-16 05:11:44ID requested
12021-03-12 19:55:31announced
Publication List
Blanken D, Foschepoth D, SerrĂ£o AC, Danelon C, Genetically controlled membrane synthesis in liposomes. Nat Commun, 11(1):4317(2020) [pubmed]
Keyword List
submitter keyword: PURE, cell free, synthetic cell, gene expression, lipid biosynthesis
Contact List
David Foschepoth
contact affiliationTU Delft
contact emaild.j.foschepoth@tudelft.nl
lab head
David Foschepoth
contact affiliationTU Delft
contact emaild.j.foschepoth@tudelft.nl
dataset submitter
Full Dataset Link List
Panorama Public dataset URI