PXD020103

PXD020103 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics analysis of Polynucleobacter asymbioticus
Description	Comparative proteomics analysis to evaluate the influence of varying culture conditions on a planktonic freshwater dwelling bacterium, Polynucleobacter asymbioticus that belongs to the class Gammaproteobacteria. In the present study, a type strain Polynucleobacter asymbioticus strain QLW-P1DMWA-1T (= DSM 18221 = CIP 10981) was cultured in triplicates in sterile UV-permeable polyethylene bags (~35% permeability; Whirl-Pak®, Nasco). Culturing was carried out at 4°C, 26°C and 26° C plus 30 minutes of ultraviolet radiation exposure two times a day using 100W ARIMED® B UV lamps (Cosmedico®, Germany). We have applied mass spectrometry based large-scale screening of proteins to investigate to better understanding of the survival nature of this microbe.
HostingRepository	PRIDE
AnnounceDate	2020-09-09
AnnouncementXML	Submission_2020-09-08_23:46:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jayaseelan Murugaiyan
SpeciesList	scientific name: Polynucleobacter asymbioticus; NCBI TaxID: 576611; scientific name: Polynucleobacter asymbioticus QLW-P1DMWA-1; NCBI TaxID: 312153;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-29 07:32:19	ID requested
⏵ 1	2020-09-08 23:46:28	announced

Dataset with its publication pending

submitter keyword: Polynucleobacter asymbioticus

Gram-negative

aerobic

freshwater habitats

proteomics

LTQ Orbitrap Velos mass spectrometer

maxquant

Perseus software

Jayaseelan Murugaiyan
contact affiliation	Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Free University of Berlin, Robert-von-Ostertag-Str. 7-13, Berlin 14163, Germany. Jayaseelan.murugaiyan@fu-berlin.de, Department of Biotechnology, SRM University AP, Andhra Pradesh, Neerukonda, Mangalagiri, Andhra Pradesh 522 502, India. jayaseelan.m@srmap.edu.in
contact email	jayaseelan.murugaiyan@fu-berlin.de
lab head
Jayaseelan Murugaiyan
contact affiliation	Freie Universitaet-Berlin,Institute of Animal Hygiene and Environmental Health
contact email	jayaseelan.murugaiyan@fu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/09/PXD020103

PRIDE project URI

• PRIDE
  1. PXD020103
     1. Label: PRIDE project
     2. Name: Comparative proteomics analysis of Polynucleobacter asymbioticus