PXD020075-1

PXD020075 is an original dataset announced via ProteomeXchange.

Title	Distinct Basket Nucleoporins roles in Nuclear Pore Function and Gene Expression
Description	Nuclear pore complexes (NPCs) are important for cellular functions beyond nucleocytoplasmic trafficking, including genome organization and gene expression. This multi-faceted nature and the slow turnover of NPC components complicates investigations of how individual nucleoporins act in these diverse processes. To address this question, we used an Auxin-Induced Degron (AID) system to distinguish roles of basket nucleoporins Nup153, Nup50 and Tpr. Here, we provide MS data for the Nuclear Pore-enriched fraction of human DLD-1 cells, expressing CRISPR-engeneered degron, fused with the endogenous locus of corresponding basket NUPs, in the absence or presence of Auxin.
HostingRepository	PRIDE
AnnounceDate	2020-06-30
AnnouncementXML	Submission_2020-06-29_23:35:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexei Arnaoutov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-29 00:02:47	ID requested
⏵ 1	2020-06-29 23:35:35	announced
2	2020-09-15 23:15:40	announced	2020-09-16: Updated publication reference for PubMed record(s): 32917881.

Dataset with its publication pending

submitter keyword: human, nuclear pore complex, auxin-inducible degron

Mary Dasso
contact affiliation	Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD/NIH) Section on Cell Cycle Regulation Building 49, Room 5A30 49 Convent Drive Bethesda, MD 20892-4480
contact email	dassom@mail.nih.gov
lab head
Alexei Arnaoutov
contact affiliation	NICHD/NIH
contact email	arnaouta@mail.nih.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD020075

PRIDE project URI

• PRIDE
  1. PXD020075
     1. Label: PRIDE project
     2. Name: Distinct Basket Nucleoporins roles in Nuclear Pore Function and Gene Expression