PXD020075 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Distinct Basket Nucleoporins roles in Nuclear Pore Function and Gene Expression |
Description | Nuclear pore complexes (NPCs) are important for cellular functions beyond nucleocytoplasmic trafficking, including genome organization and gene expression. This multi-faceted nature and the slow turnover of NPC components complicates investigations of how individual nucleoporins act in these diverse processes. To address this question, we used an Auxin-Induced Degron (AID) system to distinguish roles of basket nucleoporins Nup153, Nup50 and Tpr. Here, we provide MS data for the Nuclear Pore-enriched fraction of human DLD-1 cells, expressing CRISPR-engeneered degron, fused with the endogenous locus of corresponding basket NUPs, in the absence or presence of Auxin. |
HostingRepository | PRIDE |
AnnounceDate | 2020-06-30 |
AnnouncementXML | Submission_2020-09-15_23:13:13.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alexei Arnaoutov |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Orbitrap Fusion Lumos; LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-06-29 00:02:47 | ID requested | |
1 | 2020-06-29 23:35:35 | announced | |
⏵ 2 | 2020-09-15 23:15:40 | announced | 2020-09-16: Updated publication reference for PubMed record(s): 32917881. |
Publication List
Aksenova V, Smith A, Lee H, Bhat P, Esnault C, Chen S, Iben J, Kaufhold R, Yau KC, Echeverria C, Fontoura B, Arnaoutov A, Dasso M, Nucleoporin TPR is an integral component of the TREX-2 mRNA export pathway. Nat Commun, 11(1):4577(2020) [pubmed] |
Keyword List
submitter keyword: human, nuclear pore complex, auxin-inducible degron |
Contact List
Mary Dasso |
contact affiliation | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD/NIH) Section on Cell Cycle Regulation Building 49, Room 5A30 49 Convent Drive Bethesda, MD 20892-4480 |
contact email | dassom@mail.nih.gov |
lab head | |
Alexei Arnaoutov |
contact affiliation | NICHD/NIH |
contact email | arnaouta@mail.nih.gov |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD020075
- Label: PRIDE project
- Name: Distinct Basket Nucleoporins roles in Nuclear Pore Function and Gene Expression