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PXD020075 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDistinct Basket Nucleoporins roles in Nuclear Pore Function and Gene Expression
DescriptionNuclear pore complexes (NPCs) are important for cellular functions beyond nucleocytoplasmic trafficking, including genome organization and gene expression. This multi-faceted nature and the slow turnover of NPC components complicates investigations of how individual nucleoporins act in these diverse processes. To address this question, we used an Auxin-Induced Degron (AID) system to distinguish roles of basket nucleoporins Nup153, Nup50 and Tpr. Here, we provide MS data for the Nuclear Pore-enriched fraction of human DLD-1 cells, expressing CRISPR-engeneered degron, fused with the endogenous locus of corresponding basket NUPs, in the absence or presence of Auxin.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlexei Arnaoutov
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion Lumos; LTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-29 00:02:47ID requested
12020-06-29 23:35:35announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: human, nuclear pore complex, auxin-inducible degron
Contact List
Mary Dasso
contact affiliationEunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD/NIH) Section on Cell Cycle Regulation Building 49, Room 5A30 49 Convent Drive Bethesda, MD 20892-4480
contact emaildassom@mail.nih.gov
lab head
Alexei Arnaoutov
contact affiliationNICHD/NIH
contact emailarnaouta@mail.nih.gov
dataset submitter
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