PXD020016-1

PXD020016 is an original dataset announced via ProteomeXchange.

Title	ADAM17 cytoplasmic domain modulates Thioredoxin-1
Description	We have previously shown that Thioredoxin-1 (Trx-1) binds to the cytoplasmic domain of ADAM17 (ADAM17cyto), A Disintegrin And Metalloprotease 17, and that the destabilization in their interface of interaction favors the Trx-1 dimeric and inactive state. These studies opened the question of whether ADAM17 plays a role in the modulation of Trx-1 conformation and activity. Here, we demonstrate that site-directed mutagenesis in ADAM17 (ADAM17cytoF730A) also disrupts the interacting interface with Trx-1, resulting in a decrease of Trx-1 reductive capacity and activity. One of the mechanisms that explain this effect might be that ADAM17cyto favors Trx-1 monomerization state - the active state - by forming a disulfide bond between Cys824 at the C-terminal of ADAM17cyto with the Cys73 of Trx-1, which is involved in the dimerization site. Both the free Trx-1 Cys73 peptide and the disulfide bond Trx-1 Cys73-ADAM17 Cys824 peptide were measured by SRM.
HostingRepository	PanoramaPublic
AnnounceDate	2021-03-12
AnnouncementXML	Submission_2021-03-12_20:15:07.772.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	supported by repository but incomplete data and/or metadata
PrimarySubmitter	Adriana Paes Leme
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Xevo TQ-XS

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-25 06:29:02	ID requested
⏵ 1	2021-03-12 20:15:08	announced

E Costa RAP, Granato DC, Trino LD, Yokoo S, Carnielli CM, Kawahara R, Domingues RR, Pauletti BA, Neves LX, Santana AG, Paulo JA, Arag, ã, o AZB, Heleno Batista FA, Migliorini Figueira AC, Laurindo FRM, Fernandes D, Hansen HP, Squina F, Gygi SP, Paes Leme AF, ADAM17 cytoplasmic domain modulates Thioredoxin-1 conformation and activity. Redox Biol, 37():101735(2020) [pubmed]

submitter keyword: Trx-1 ADAM17 disulfide bond shuffle

Adriana Paes Leme
contact affiliation	CNPEM
contact email	adriana.paesleme@lnbio.cnpem.br
lab head
Adriana Paes Leme
contact affiliation	CNPEM
contact email	adriana.paesleme@lnbio.cnpem.br
dataset submitter

Panorama Public dataset URI