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PXD019791 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOrdered dephosphorylation initiated by the selective proteolysis of cyclin B drives mitotic exit
DescriptionAPC/C-mediated proteolysis of cyclin B and securin promotes entry into anaphase, inactivating CDK1 and permitting chromosome segregation, respectively. Reduction of CDK1 activity relieves inhibition of the CDK1-opposing phosphatases PP1 and PP2A-B55 leading to dephosphorylation of substrates crucial for mitotic exit. Meanwhile, continued APC/C activity is required to target various proteins, including Aurora and Polo kinases, for degradation. Together, these activities orchestrate a complex series of events during mitotic exit. However, the relative importance of regulated proteolysis and dephosphorylation in dictating the order and timing of these events remains unclear. Using high temporal-resolution mass spectrometry, we compare the relative extent of proteolysis and protein dephosphorylation. This reveals highly-selective rapid (~5min half-life) proteolysis of cyclin B, securin and geminin at the metaphase to anaphase transition, followed by slow proteolysis (>60 min half-life) of other mitotic regulators. Protein dephosphorylation requires APC/C-dependent destruction of cyclin B and was resolved into PP1-dependent fast, intermediate and slow categories with unique sequence motifs. We conclude that dephosphorylation initiated by the selective proteolysis of cyclin B drives the bulk of changes observed during mitotic exit.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJames Holder
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-15 07:36:59ID requested
12020-09-04 04:33:06announced
Publication List
Keyword List
submitter keyword: Mitosis, mitotic exit, cell cycle, proteomics, proteolysis, APC/C
Contact List
Francis Barr
contact affiliationDepartment of Biochemsitry, University of Oxford
contact emailfrancis.barr@bioch.ox.ac.uk
lab head
James Holder
contact affiliationUniversity of Oxford
contact emailjames.holder@path.ox.ac.uk
dataset submitter
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