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PXD019681 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhage Phix174 - Wildtype and engineered proteome
DescriptionSequence overlap between two genes is common across all genomes, with viruses having particularly high proportions of these gene overlaps. The natural biological function and effects on fitness of gene overlaps are not fully understood and their effects on gene cluster and genome-level refactoring are unknown.The model bacteriophage φX174 genome displays complex sequence architecture in which ~26% of nucleotides are involved in encoding more than one gene. In this study we use an engineered φX174 phage containing a genome with all gene overlaps removed. Here we have temporally measured the proteome of a synthetically engineered and wild-type φX174 during infection. We find that almost half of all phage proteins (5/11) have abnormal expression profiles after genome modularisation.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterBradley Wright
SpeciesList scientific name: synthetic Enterobacteria phage phiX174.1f; NCBI TaxID: 1246486; scientific name: Escherichia virus phiX174; NCBI TaxID: 10847;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-09 15:08:02ID requested
12021-06-15 07:00:29announced
Publication List
Wright BW, Ruan J, Molloy MP, Jaschke PR, Genome Modularization Reveals Overlapped Gene Topology Is Necessary for Efficient Viral Reproduction. ACS Synth Biol, 9(11):3079-3090(2020) [pubmed]
Keyword List
submitter keyword: Overlapping genes, bacteriophage, virus
Contact List
Paul Jaschke
contact affiliationMacquarie University, Department of Molecular Sciences
contact emailpaul.jaschke@mq.edu.au
lab head
Bradley Wright
contact affiliationMacquarie University, Department of Molecular Sciences
contact emailbradley.wright@students.mq.edu.au
dataset submitter
Full Dataset Link List
Panorama Public dataset URI