PXD019654-1
PXD019654 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Methyl-SILAF mapping of the Drosophila mitochondrial protein methylome |
Description | S-adenosylmethionine (SAM) is the principal biological methyl group donor for a diverse range of substrates. It is synthesised in the cytosolic methionine cycle and shuttled throughout the cell. The mitochondrial SAM (mitoSAM) pool depends on import through the inner-membrane S-adenosylmethionine carrier (SAMC) and supports the maturation of metabolites and mitochondrial RNAs. Mutations in SAMC in patients cause a severe metabolic crisis, however, the organellar regulation of mitoSAM and the protein methylation landscape within mitochondria are largely unknown. We developed a fly-compatible SILAC labelling technique and mapped mitochondrial protein methylation sites in Drosophila melanogaster, further showing that SAM is the sole methyl group donor for these modifications, with no contribution from folate-species. This dataset comprises MS-runs used for quality control of methyl-SILAF, the methylome mapping and exclusion of folates as protein methyl-group donors. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-22 |
AnnouncementXML | Submission_2021-02-22_06:15:19.215.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Florian Schober |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | Methyl:2H(3)13C(1); iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos; Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-06-08 07:46:01 | ID requested | |
⏵ 1 | 2021-02-22 06:15:20 | announced |
Publication List
Rosenberger FA, Moore D, Atanassov I, Moedas MF, Clemente P, V, é, gv, á, ri Á, Fissi NE, Filograna R, Bucher AL, Hinze Y, The M, Hedman E, Chernogubova E, Begzati A, Wibom R, Jain M, Nilsson R, K, ä, ll L, Wedell A, Freyer C, Wredenberg A, The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clusters. Sci Adv, 7(8):(2021) [pubmed] |
Keyword List
submitter keyword: Methylation, mitochondria, PTM, Drosophila |
Contact List
Anna Wredenberg | |
---|---|
contact affiliation | Department of Medical Biochemistry and Biophysics, Karolinska Institute, Sweden |
contact email | anna.wredenberg@ki.se |
lab head | |
Florian Schober | |
contact affiliation | MMK, Karolinska Institute |
contact email | florian.schober@ki.se |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD019654 |
PRIDE project URI |
Repository Record List
[ + ]