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PXD019654 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMethyl-SILAF mapping of the Drosophila mitochondrial protein methylome
DescriptionS-adenosylmethionine (SAM) is the principal biological methyl group donor for a diverse range of substrates. It is synthesised in the cytosolic methionine cycle and shuttled throughout the cell. The mitochondrial SAM (mitoSAM) pool depends on import through the inner-membrane S-adenosylmethionine carrier (SAMC) and supports the maturation of metabolites and mitochondrial RNAs. Mutations in SAMC in patients cause a severe metabolic crisis, however, the organellar regulation of mitoSAM and the protein methylation landscape within mitochondria are largely unknown. We developed a fly-compatible SILAC labelling technique and mapped mitochondrial protein methylation sites in Drosophila melanogaster, further showing that SAM is the sole methyl group donor for these modifications, with no contribution from folate-species. This dataset comprises MS-runs used for quality control of methyl-SILAF, the methylome mapping and exclusion of folates as protein methyl-group donors.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFlorian Schober
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListMethyl:2H(3)13C(1); iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos; Q Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-08 07:46:01ID requested
12021-02-22 06:15:20announced
Publication List
Schober FA, Moore D, Atanassov I, Moedas MF, Clemente P, Végvári Á, Fissi NE, Filograna R, Bucher AL, Hinze Y, The M, Hedman E, Chernogubova E, Begzati A, Wibom R, Jain M, Nilsson R, Käll L, Wedell A, Freyer C, Wredenberg A, The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clusters. Sci Adv, 7(8):(2021) [pubmed]
Keyword List
submitter keyword: Methylation, mitochondria, PTM, Drosophila
Contact List
Anna Wredenberg
contact affiliationDepartment of Medical Biochemistry and Biophysics, Karolinska Institute, Sweden
contact emailanna.wredenberg@ki.se
lab head
Florian Schober
contact affiliationMMK, Karolinska Institute
contact emailflorian.schober@ki.se
dataset submitter
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