PXD019654

PXD019654 is an original dataset announced via ProteomeXchange.

Title	Methyl-SILAF mapping of the Drosophila mitochondrial protein methylome
Description	S-adenosylmethionine (SAM) is the principal biological methyl group donor for a diverse range of substrates. It is synthesised in the cytosolic methionine cycle and shuttled throughout the cell. The mitochondrial SAM (mitoSAM) pool depends on import through the inner-membrane S-adenosylmethionine carrier (SAMC) and supports the maturation of metabolites and mitochondrial RNAs. Mutations in SAMC in patients cause a severe metabolic crisis, however, the organellar regulation of mitoSAM and the protein methylation landscape within mitochondria are largely unknown. We developed a fly-compatible SILAC labelling technique and mapped mitochondrial protein methylation sites in Drosophila melanogaster, further showing that SAM is the sole methyl group donor for these modifications, with no contribution from folate-species. This dataset comprises MS-runs used for quality control of methyl-SILAF, the methylome mapping and exclusion of folates as protein methyl-group donors.
HostingRepository	PRIDE
AnnounceDate	2021-02-22
AnnouncementXML	Submission_2021-02-22_06:15:19.215.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Florian Schober
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	Methyl:2H(3)13C(1); iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-08 07:46:01	ID requested
⏵ 1	2021-02-22 06:15:20	announced

Rosenberger FA, Moore D, Atanassov I, Moedas MF, Clemente P, V, é, gv, á, ri Á, Fissi NE, Filograna R, Bucher AL, Hinze Y, The M, Hedman E, Chernogubova E, Begzati A, Wibom R, Jain M, Nilsson R, K, ä, ll L, Wedell A, Freyer C, Wredenberg A, The one-carbon pool controls mitochondrial energy metabolism via complex I and iron-sulfur clusters. Sci Adv, 7(8):(2021) [pubmed]

submitter keyword: Methylation, mitochondria, PTM, Drosophila

Anna Wredenberg
contact affiliation	Department of Medical Biochemistry and Biophysics, Karolinska Institute, Sweden
contact email	anna.wredenberg@ki.se
lab head
Florian Schober
contact affiliation	MMK, Karolinska Institute
contact email	florian.schober@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD019654

PRIDE project URI

• PRIDE
  1. PXD019654
     1. Label: PRIDE project
     2. Name: Methyl-SILAF mapping of the Drosophila mitochondrial protein methylome