PXD019645

PXD019645 is an original dataset announced via ProteomeXchange.

Title	Data, reagents, assays and merits of proteomics for SARS-CoV-2 research and testing
Description	As the SARS-CoV-2 pandemic continues to spread, thousands of scientists around the globe have changed research direction to understand better how the virus works and to find out how it may be tackled. To facilitate proteomic research on SARS-CoV-2, we presents deep-scale proteomes of common cell line models (Calu-3, Caco-2, ACE2 transfected A549 and Vero E6) and monitors changes of the proteome upon viral infection in Vero E6 cells. We provide high quality proteome expression data that can be used to refine the annotation of protein coding regions of the Vero E6 cell line genome. Further, we generated spectral libraries that can be used DIA cell line experiments or PRM assays of SARS-CoV-2 proteins.
HostingRepository	PRIDE
AnnounceDate	2020-06-30
AnnouncementXML	Submission_2020-06-30_04:05:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chien-Yun Lee
SpeciesList	scientific name: Cercopithecus aethiops (Green monkey) (Grivet); NCBI TaxID: 9534; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Chlorocebus sabaeus; NCBI TaxID: 60711;
ModificationList	monohydroxylated residue; N-acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-06-08 04:54:13	ID requested
1	2020-06-28 23:18:01	announced
⏵ 2	2020-06-30 04:05:46	announced	2020-06-30: Updated publication reference for PubMed record(s): 32591346. 2020-06-30: Updated publication reference for DOI(s): 10.1074/mcp.RA120.002164.

10.1074/mcp.RA120.002164;

Zecha J, Lee CY, Bayer FP, Meng C, Grass V, Zerweck J, Schnatbaum K, Michler T, Pichlmair A, Ludwig C, Kuster B, Data, Reagents, Assays and Merits of Proteomics for SARS-CoV-2 Research and Testing. Mol Cell Proteomics, 19(9):1503-1522(2020) [pubmed]

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: SARS-CoV-2, stable isotope labeling, parallel reaction monitoring, Vero E6, ACE2, infectome, COVID-19, clinical proteomics

Bernhard Kuster
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich, Germany
contact email	kuster@tum.de
lab head
Chien-Yun Lee
contact affiliation	Chair of Proteomics and Bioanalytics, Technical University of Munich (TUM)
contact email	chienyun.lee@tum.de
dataset submitter

Dataset FTP location

PRIDE project URI

• PRIDE
  1. PXD019645
     1. Label: PRIDE project
     2. Name: Data, reagents, assays and merits of proteomics for SARS-CoV-2 research and testing