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PXD019492-1

PXD019492 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSWATH-MS proteomic analysis of liver samples prepared from liver-specific HyD-LIR expressing mice.
DescriptionHyD-LIR-Venus consists of HyD, a short hydrophobic domain of Aplysia phosphodiesterase 4 short-form; LIR of TP53INP2; and a fluorescent protein, Venus. This protein efficiently binds to LC3-II and GABARAP-II localizing on inner membrane of autophagosomes and does not affect autophagosome formation.Liver samples prepared from liver-specific HyD-LIR expressing mice together with the control mice were analyzed by mass spectrometry (TripleTOF 5600) coupled to Ekspert nanoLC 415 system with Ekspert cHipLC (Sciex, Framingham, MA). The analysis results were searched against the UniProt database (release 2019_11) using ProteinPilot 5.0.2. software (Sciex). The library contained 2619 protein groups with the cutoff of 1% false discovery rate.
HostingRepositoryjPOST
AnnounceDate2020-11-09
AnnouncementXMLSubmission_2020-11-08_22:15:19.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYoshiki Miura
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListS-carboxymethyl-L-cysteine; N6-acetyl-L-lysine; alpha-amino acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); 2-pyrrolidone-5-carboxylic acid (Glu); L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-06-01 06:10:56ID requested
12020-11-08 22:15:20announced
22021-02-04 16:39:11announced2021-02-04: Updated FTP location.
32022-09-18 02:49:18announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Autophagy, Selective autophagy, LC3, GABARAP
Contact List
Yoshiki Miura
lab head
Yoshiki Miura
contact affiliationLaboratory of Proteomics and Biomolecular Science, Research Support Center, Juntendo University Graduate School of Medicine
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://jpost.pharm.kyoto-u.ac.jp/repository/JPST000830