PXD019459-1
PXD019459 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Quantitative nascent proteome profiling by pulse labeling of O-propargyl-puromycin and stable isotope labeled amino acids |
Description | Recently, a clickable puromycin analog, O-propargyl-puromycin (OPP), was developed and applied to label C-termini of nascent polypeptide chains (NPCs), followed by a proteomic analysis of NPCs that can be affinity purified via click reaction. Despite its advantage, the affinity purification of NPCs using magnetic beads or resins inherently suffers from dozens of non-specific protein binding, which results in hindering accurate quantification of the nascent proteins. To address this issue, we performed dual pulse labeling of NPCs with both OPP and stable isotope labeled amino acids that allows us to distinguish bona fide NPCs from non-specific proteins, thereby enabling accurate quantitative profiling of NPCs. |
HostingRepository | jPOST |
AnnounceDate | 2020-09-17 |
AnnouncementXML | Submission_2020-09-16_22:54:27.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Koshi Imami |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide |
Instrument | instrument |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-05-28 22:44:17 | ID requested | |
⏵ 1 | 2020-09-16 22:54:28 | announced | |
2 | 2021-02-04 17:27:53 | announced | 2021-02-04: Updated FTP location. |
3 | 2022-09-18 04:03:36 | announced | 2022-09-18: Updated FTP location. |
Publication List
Uchiyama J, Ishihama Y, Imami K, Quantitative nascent proteome profiling by dual-pulse labelling with O-propargyl-puromycin and stable isotope-labelled amino acids. J Biochem, 169(2):227-236(2021) [pubmed] |
Keyword List
submitter keyword: OPP, pSILAC, ribosome, nascent polypeptide chain, translation |
Contact List
Yasushi Ishihama | |
---|---|
lab head | |
Koshi Imami | |
contact affiliation | Kyoto University |
dataset submitter |
Full Dataset Link List
jPOST dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://jpost.pharm.kyoto-u.ac.jp/repository/JPST000860 |