PXD019459

PXD019459 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Quantitative nascent proteome profiling by pulse labeling of O-propargyl-puromycin and stable isotope labeled amino acids
Description	Recently, a clickable puromycin analog, O-propargyl-puromycin (OPP), was developed and applied to label C-termini of nascent polypeptide chains (NPCs), followed by a proteomic analysis of NPCs that can be affinity purified via click reaction. Despite its advantage, the affinity purification of NPCs using magnetic beads or resins inherently suffers from dozens of non-specific protein binding, which results in hindering accurate quantification of the nascent proteins. To address this issue, we performed dual pulse labeling of NPCs with both OPP and stable isotope labeled amino acids that allows us to distinguish bona fide NPCs from non-specific proteins, thereby enabling accurate quantitative profiling of NPCs.
HostingRepository	jPOST
AnnounceDate	2020-09-17
AnnouncementXML	Submission_2022-09-18_04:03:36.084.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Koshi Imami
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-28 22:44:17	ID requested
1	2020-09-16 22:54:28	announced
2	2021-02-04 17:27:53	announced	2021-02-04: Updated FTP location.
⏵ 3	2022-09-18 04:03:36	announced	2022-09-18: Updated FTP location.

Publication List

Uchiyama J, Ishihama Y, Imami K, Quantitative nascent proteome profiling by dual-pulse labelling with O-propargyl-puromycin and stable isotope-labelled amino acids. J Biochem, 169(2):227-236(2021) [pubmed]

Uchiyama J, Ishihama Y, Imami K, Quantitative nascent proteome profiling by dual-pulse labelling with O-propargyl-puromycin and stable isotope-labelled amino acids. J Biochem, 169(2):227-236(2021) [pubmed]

Keyword List

submitter keyword: OPP, pSILAC, ribosome, nascent polypeptide chain, translation

submitter keyword: OPP, pSILAC, ribosome, nascent polypeptide chain, translation

Contact List

Yasushi Ishihama
lab head
Koshi Imami
contact affiliation	Kyoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000860/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000860/