PXD019350 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A human tissue screen identifies a regulator of ER secretion as a brain size determinant |
Description | A human tissue screen identifies a regulator of ER secretion as a brain size determinant. Abstract: Loss-of-function (LOF) screens provide a powerful approach to identify regulators in biological processes. Pioneered in laboratory animals, LOF screens of human genes are currently restricted to two-dimensional (2D) cell culture hindering testing of gene functions requiring tissue context. Here we present CRISPR-LIneage tracing at Cellular resolution in Heterogenous Tissue (CRISPR-LICHT), enabling parallel LOF studies in human cerebral organoid tissue. We used CRISPR-LICHT to test 173 microcephaly candidate genes revealing 25 to be involved in known and uncharacterized microcephaly-associated pathways. We characterized Immediate Early Response 3 Interacting Protein 1 (IER3IP1) regulating the unfolded protein response (UPR) and extracellular matrix (ECM) protein secretion crucial for tissue integrity, with dysregulation resulting in microcephaly. Our human tissue screening technology identifies microcephaly genes and mechanisms involved in brain size control. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-10 |
AnnouncementXML | Submission_2021-11-10_06:01:45.164.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Richard Imre |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | carbamoylated residue; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-22 05:33:30 | ID requested | |
⏵ 1 | 2021-11-10 06:01:45 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Organoids, screen, IER3IP1, UPR, ECM |
Contact List
Juergen Knoblich |
contact affiliation | IMBA - Institute of Molecular Biotechnology GmbH Dr. Bohr-Gasse 3 1030 Vienna Austria |
contact email | Juergen.Knoblich@imba.oeaw.ac.at |
lab head | |
Richard Imre |
contact affiliation | IMBA - Institute of Molecular Biotechnology |
contact email | imre@imp.ac.at |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD019350
- Label: PRIDE project
- Name: A human tissue screen identifies a regulator of ER secretion as a brain size determinant