PXD019306 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic analysis of the trypanosomatid Novymonas esmeraldas |
Description | An endosymbiotic event where an archaeon acquired and intracellular protobacteria underpinned the evolution of mitochondria, a key event for the evolution of eukaryotes. Despite is biological importance, the molecular mechanisms driving the early stages of the host-endosymbiont interactions that lead to the evolution of endosymbiont-derived organelles remains poorly understood. Novymonas esmeraldas is a trypanosomatid that recently acquired a -proteobacterial endosymbiont which is compartmentalized into vacuole-like structures called symbiosomes. The life cycle of the host is not synchronized with that of its endosymbiont resulting in heterogeneous numbers of endosymbionts per cell among the population. In order to elucidate the molecular mechanisms mediating this early stage of the host-endosymbiont interactions we isolated the endosymbionts from Novymonas esmeraldas and analyzed the proteome of isolated endosymbionts, holosymbionts (cells containing endosymbionts) and aposymbionts (cells where endosymbionts where removed by treatment with antibiotics). We show that at least one host-encoded protein localizes to the symbiosome and knockout of the corresponding gene leads to an increase of over 2-fold of endosymbionts per cell. This result suggests that controlling the endosymbiont proliferation by the host is an early step for the establishment and posterior evolution of an endosymbiotic event. |
HostingRepository | PRIDE |
AnnounceDate | 2023-05-22 |
AnnouncementXML | Submission_2023-05-22_01:58:09.426.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | GereonPoschmann |
SpeciesList | scientific name: Pandoraea; NCBI TaxID: 93217; scientific name: cellular organisms; NCBI TaxID: NCBITaxon:131567; scientific name: Novymonas esmeraldas; NCBI TaxID: 1808958; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-05-20 04:18:55 | ID requested | |
⏵ 1 | 2023-05-22 01:58:10 | announced | |
2 | 2023-11-14 08:48:10 | announced | 2023-11-14: Updated project metadata. |
Publication List
Zakharova A, Tashyreva D, Butenko A, Morales J, Saura A, Svobodov, á M, Poschmann G, Nandipati S, Zakharova A, Noyvert D, Gahura O, T, ý, č J, St, ü, hler K, Kostygov AY, Nowack ECM, Luke, š J, Yurchenko V, A neo-functionalized homolog of host transmembrane protein controls localization of bacterial endosymbionts in the trypanosomatid Novymonas esmeraldas. Curr Biol, 33(13):2690-2701.e5(2023) [pubmed] |
Keyword List
submitter keyword: evolution, eukaryotes, mitochondria, protobacteria,endosymbiont, Novymonas esmeraldas |
Contact List
GereonPoschmann |
contact affiliation | Proteome Research (AG Stühler) Institute of Molecular Medicine I Heinrich Heine University Düsseldorf Germany |
contact email | gereon.poschmann@hhu.de |
lab head | |
GereonPoschmann |
contact affiliation | Molecular Proteomics Laboratory |
contact email | gereon.poschmann@hhu.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD019306
- Label: PRIDE project
- Name: Proteomic analysis of the trypanosomatid Novymonas esmeraldas