PXD019306

PXD019306 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the trypanosomatid Novymonas esmeraldas
Description	An endosymbiotic event where an archaeon acquired and intracellular protobacteria underpinned the evolution of mitochondria, a key event for the evolution of eukaryotes. Despite is biological importance, the molecular mechanisms driving the early stages of the host-endosymbiont interactions that lead to the evolution of endosymbiont-derived organelles remains poorly understood. Novymonas esmeraldas is a trypanosomatid that recently acquired a -proteobacterial endosymbiont which is compartmentalized into vacuole-like structures called symbiosomes. The life cycle of the host is not synchronized with that of its endosymbiont resulting in heterogeneous numbers of endosymbionts per cell among the population. In order to elucidate the molecular mechanisms mediating this early stage of the host-endosymbiont interactions we isolated the endosymbionts from Novymonas esmeraldas and analyzed the proteome of isolated endosymbionts, holosymbionts (cells containing endosymbionts) and aposymbionts (cells where endosymbionts where removed by treatment with antibiotics). We show that at least one host-encoded protein localizes to the symbiosome and knockout of the corresponding gene leads to an increase of over 2-fold of endosymbionts per cell. This result suggests that controlling the endosymbiont proliferation by the host is an early step for the establishment and posterior evolution of an endosymbiotic event.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:09.271.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gereon Poschmann
SpeciesList	scientific name: Pandoraea; NCBI TaxID: 93217; scientific name: cellular organisms; NCBI TaxID: NCBITaxon:131567; scientific name: Novymonas esmeraldas; NCBI TaxID: 1808958;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-20 04:18:55	ID requested
1	2023-05-22 01:58:10	announced
⏵ 2	2023-11-14 08:48:10	announced	2023-11-14: Updated project metadata.

Zakharova A, Tashyreva D, Butenko A, Morales J, Saura A, Svobodov, á M, Poschmann G, Nandipati S, Zakharova A, Noyvert D, Gahura O, T, ý, č J, St, ü, hler K, Kostygov AY, Nowack ECM, Luke, š J, Yurchenko V, A neo-functionalized homolog of host transmembrane protein controls localization of bacterial endosymbionts in the trypanosomatid Novymonas esmeraldas. Curr Biol, 33(13):2690-2701.e5(2023) [pubmed]

submitter keyword: evolution, eukaryotes, mitochondria, protobacteria,endosymbiont, Novymonas esmeraldas

Gereon Poschmann
contact affiliation	Proteome Research (AG Stühler) Institute of Molecular Medicine I Heinrich Heine University Düsseldorf Germany
contact email	gereon.poschmann@hhu.de
lab head
Gereon Poschmann
contact affiliation	Molecular Proteomics Laboratory
contact email	gereon.poschmann@hhu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD019306

PRIDE project URI

• PRIDE
  1. PXD019306
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the trypanosomatid Novymonas esmeraldas