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PXD019300-3

PXD019300 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSARS-CoV-2_PRM_SRM_targeted_acquisitions
DescriptionThe current outbreak of severe acute respiratory syndrome associated with coronavirus 2 (SARS-CoV-2) is pressing public health systems around the world, and large population testing is a key step to control this pandemic disease. We developed a high-throughput targeted proteomics assay to detect SARS-CoV-2 proteins directly from nasopharyngeal and oropharyngeal swabs. Sample preparation was fully automated by using a modified magnetic particle-based proteomics approach implemented on a robotic liquid handler. The use of turbulent flow chromatography included four times multiplexed on-line sample cleanup and liquid chromatography separation. Mass spectrometry detection of nucleoprotein peptides was achieved within 2.5 min, enabling the analysis of more than 500 samples per day. The method was validated qualitatively (Tier 3) and quantitatively (Tier 1) using 855 specimens previously analyzed by real-time RT-PCR and was able to detect up to 84% of positive cases with up to 97% of specificity. The strategy here presented has high sample stability and should be considered as an option for testing in large populations.
HostingRepositoryPanoramaPublic
AnnounceDate2020-12-07
AnnouncementXMLSubmission_2020-12-07_13:37:09.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterValdemir Carvalho
SpeciesList scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationListLabel:15N(3); Label:15N(1); Label:15N(2)
InstrumentQ Exactive HF-X; TSQ Altis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-19 20:28:41ID requested
12020-05-20 20:16:54announced
22020-09-16 09:39:17announced: Added quantitative validation experiments
32020-12-07 13:37:10announced: Added PubMed ID
Publication List
Cardozo KHM, Lebkuchen A, Okai GG, Schuch RA, Viana LG, Olive AN, Lazari CDS, Fraga AM, Granato CFH, Pint, ã, o MCT, Carvalho VM, Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts. Nat Commun, 11(1):6201(2020) [pubmed]
Keyword List
submitter keyword: SARS-CoV-2, COVID-19, targeted proteomics
Contact List
Valdemir Carvalho
contact affiliationDivision of Research and Development, Fleury Group, 04344-070, São Paulo, SP, Brazil
contact emailmelechco@gmail.com
lab head
Valdemir Carvalho
contact affiliationDivision of Research and Development, Fleury Group, 04344-070, São Paulo, SP, Brazil
contact emailmelechco@gmail.com
dataset submitter
Full Dataset Link List
Panorama Public dataset URI