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PXD019300

PXD019300 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSARS-CoV-2_PRM_SRM_targeted_acquisitions
DescriptionWe present here a high-throughput targeted proteomics assay to detect SARS-CoV-2 proteins directly from clinical respiratory tract samples. The use of turbulent flow chromatography included four times multiplexed on-line sample cleanup and UPLC separation. MS/MS detection of three peptides from SARS-CoV-2 nucleoprotein and a 15N-labeled internal global standard was achieved within 2.5 min, enabling the analysis of more than 500 samples per day. The method was validated using 562 specimens previously analyzed by real-time RT-PCR and was able to detect over 83% of positive cases. No interference was found with samples from common respiratory viruses, including other coronaviruses (NL63, OC43, HKU1, and 229E). In addition to the SRM method targeted to 3 peptides, two PRM methods used for peptides selection are also described. The strategy here presented has high sample stability and lower cost compared to real-time RT-PCR and could be considered as an option to large population SARS-CoV-2 testing.
HostingRepositoryPanoramaPublic
AnnounceDate2020-05-20
AnnouncementXMLSubmission_2020-05-20_20:16:53.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterValdemir Carvalho
SpeciesList scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049;
ModificationListLabel:15N(3); Label:15N(1); Label:15N(2)
InstrumentQ Exactive HF-X; TSQ Altis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-19 20:28:41ID requested
12020-05-20 20:16:54announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SARS-CoV-2, COVID-19, targeted proteomics
Contact List
Valdemir Carvalho
contact affiliationFleury Group
contact emailmelechco@gmail.com
lab head
Valdemir Carvalho
contact affiliationFleury Group
contact emailmelechco@gmail.com
dataset submitter
Full Dataset Link List
Panorama Public dataset URI