PXD019163-1

PXD019163 is an original dataset announced via ProteomeXchange.

Title	Glycosylation analysis of SARS-CoV-2 S and hCoV-HKU1 S
Description	The rapid global spread of SARS-CoV-2 and resultant mortality and social disruption have highlighted the need to better understand coronaviral immunity to expedite vaccine development efforts. Multiple candidate vaccines with the goal of eliciting protective neutralising antibodies targeting the viral spike glycoprotein are rapidly advancing to clinical trial. However, the immunogenic properties of the spike in human populations are unresolved. To address this, we undertook an in-depth characterisation of humoral and cellular immunity against SARS-CoV-2 spike in humans following mild to moderate SARS-CoV-2 infection. We find serological antibody responses against spike are routinely elicited by infection and correlate with plasma neutralising and ACE2 inhibitory activity. Expanded populations of spike-specific memory B cells and circulating T follicular helper cells (cTFH) were detected within convalescent donors, while responses to receptor binding domain constitute a minor fraction. Using regression analysis, we find high serum neutralisation activity was associated with increased spike-specific antibody, but notably also with the relative distribution of some spike-specific cTFH subsets. Thus both qualitative and quantitative features of B and T cell immunity to spike constitute informative biomarkers to assess protective potential of novel SARS-CoV-2 vaccines
HostingRepository	PRIDE
AnnounceDate	2020-06-18
AnnouncementXML	Submission_2020-06-17_22:15:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nichollas Scott
SpeciesList	scientific name: Coronavirus PREDICT CoV-19; NCBI TaxID: 2042545;
ModificationList	complex glycosylation
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-13 08:30:31	ID requested
⏵ 1	2020-06-17 22:15:24	announced

Dataset with its publication pending

ProteomeXchange project tag: Sars-cov-2

submitter keyword: COVID19, glycoproteins

Adam Wheatley
contact affiliation	Department of Microbiology and Immunology, University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne 3000, Australia
contact email	a.wheatley@unimelb.edu.au
lab head
Nichollas Scott
contact affiliation	University of Melbourne
contact email	nichollas.scott@unimelb.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD019163

PRIDE project URI

• PRIDE
  1. PXD019163
     1. Label: PRIDE project
     2. Name: Glycosylation analysis of SARS-CoV-2 S and hCoV-HKU1 S