PXD019061-1

PXD019061 is an original dataset announced via ProteomeXchange.

Title	Analysis of Immune Checkpoint Drug Targets and Tumor Proteotypes in Non-Small Cell Lung Cancer
Description	New therapeutics targeting immune checkpoint proteins have significantly advanced treatment of non-small cell lung cancer (NSCLC), but protein level quantitation of drug targets presents a critical problem. We used multiplexed, targeted mass spectrometry (MS) to quantify the immunotherapy target proteins PD-1, PD-L1, PD-L2, IDO1, LAG3, TIM-3, VISTA, GITR, and CD40 in formalin-fixed, paraffin-embedded (FFPE) NSCLC specimens. Immunohistochemistry (IHC) and MS measurements for PD-L1 were weakly correlated, but IHC did not distinguish protein abundance differences detected by MS. PD-L2 abundance exceeded PD-L1 in over half the specimens and the drug target proteins all displayed different abundance patterns. mRNA correlated with protein abundance only for PD-1, PD-L1, and IDO1 and tumor mutation burden did not predict abundance of any protein targets. Global proteome analyses identified distinct proteotypes associated with high PD-L1-expressing and high IDO1-expressing NSCLC. MS quantification of multiple drug targets and tissue proteotypes can improve clinical evaluation of immunotherapies for NSCLC.
HostingRepository	PanoramaPublic
AnnounceDate	2020-06-22
AnnouncementXML	Submission_2020-06-22_05:42:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ryan Morrison
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-05-08 15:45:17	ID requested
⏵ 1	2020-06-22 05:42:48	announced

Liebler DC, Holzer TR, Haragan A, Morrison RD, O'Neill Reising L, Ackermann BL, Fill JA, Schade AE, Gruver AM, Analysis of Immune Checkpoint Drug Targets and Tumor Proteotypes in Non-Small Cell Lung Cancer. Sci Rep, 10(1):9805(2020) [pubmed]

submitter keyword: FFPE NSCLC PRM

Daniel Liebler
contact affiliation	Protypia, Inc
contact email	daniel.liebler@protypia.com
lab head
Ryan Morrison
contact affiliation	Protypia, Inc
contact email	ryan.morrison@protypia.com
dataset submitter

Panorama Public dataset URI