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PXD018986-1

PXD018986 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe effect of pathogen inactivation on cryoprecipitate: A functional and quantitative evaluation
DescriptionAs a pooled donor blood product, cryoprecipitate carries higher risks of pathogen transmission. Pathogen inactivation (PI) improves the safety of cryoprecipitate, but its effects on hemostatic properties remain unclear. This study investigated protein expression in samples of pathogen inactivated cryoprecipitate (PI-cryo) using non-targeted quantitative proteomics. Specifically, cryoprecipitate samples were reduced and alkylated and digested with trypsin. Approximately 50 micrograms of tryptic digests were analyzed by microflowLC-MS/MS. The LC separation used a Waters ACQUITY UPLC and a 1 mm x 150 mm 1.7 micron CSH C18 column using a flow rate of 100 microliters/min, a column temperature of 55C and a gradient of 3-28% (v/v) MeCN:H2O containing 0.1% formic acid over 60 minutes. The LC was interfaced to a Thermo Q-Exactive HF-X using the HESI-II source. The MS analysis used data-independent acquisition (DIA)-MS with a 120,000 resolution precursor ion (MS1) scan from 375-1500 m/z, AGC target of 3E6 and maximum injection time (IT) of 20 ms. DIA-MS/MS was performed using 30,000 resolution, AGC target of 3e6 and maximum IT of 60 ms, and 27 V NCE. The DIA windows included 15 x 37 m/z windows spanning 400-941 m/z with 1 m/z overlaps; as well as 3 x 87 m/z windows spanning 941-1200 m/z. Data was analyzed using Spectronaut Pulsar X
HostingRepositoryMassIVE
AnnounceDate2020-05-19
AnnouncementXMLSubmission_2020-05-19_07:01:20.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMatt Foster
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListiodoacetamide derivatized residue
Instrumentinstrument model
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-05-04 21:07:31ID requested
12020-05-19 07:01:20announced
Publication List
no publication
Keyword List
submitter keyword: plasma, apheresis, cryoprecipiate, microflow, data-independent acquisition
Contact List
Reed Kamyszek
contact affiliationUniversity of Michigan
contact emailrekamysz@med.umich.edu
lab head
Matthew W. Foster
contact affiliationDuke University
contact emailmwfoster@duke.edu
lab head
Matt Foster
contact affiliationDuke University
contact emailmwfoster@duke.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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