PXD018874-1

PXD018874 is an original dataset announced via ProteomeXchange.

Title	High-throughput proteomics platform for clinical applications
Description	Clinical proteomics holds the promise to accelerate the discovery of disease biomarkers, as well as to predict disease trajectories. Here we present a new platform for high-throughput plasma and serum proteomics, which combines an automated sample preparation workflow, robust high-flow liquid chromatography, and an optimized SWATH-MS acquisition scheme as well as data processing workflow. The process requires as little as 5uL serum or plasma and makes use of fast 5-minute chromatographic gradients. The dataset shows the robustness and precision of the workflow and consists of commercial plasma and serum samples that were distributed in 4 different 96 well plates and injected within a sample series of 417 serum injections. Further, the dataset includes of injections of a single sample (pooled from 32 prepared commercial serum samples) every 10 injections.
HostingRepository	PRIDE
AnnounceDate	2020-06-09
AnnouncementXML	Submission_2020-06-09_06:23:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christoph Messner
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2020-04-28 16:51:02	ID requested
⏵ 1	2020-06-09 06:23:03	announced

Dataset with its publication pending

submitter keyword: High-throughput proteomics, plasma, serum, clinical proteomics

Markus Ralser
contact affiliation	Charite- Universitaetsmedizin Berlin, 10117 Berlin, Germany
contact email	markus.ralser@charite.de
lab head
Christoph Messner
contact affiliation	Francis Crick Institute
contact email	christoph.messner@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/06/PXD018874

PRIDE project URI

• PRIDE
  1. PXD018874
     1. Label: PRIDE project
     2. Name: High-throughput proteomics platform for clinical applications